Neurotrophins promotes brain metastasis of triple negative breast cancer through Src kinase family activation
Julien Cicero
(1, 2, 3)
,
Sarah Trouvilliez
(1)
,
Martine Palma
(1)
,
Gaetan Ternier
(4, 5)
,
Laurine Decoster
(4, 5)
,
Nicolas Barrois
(6)
,
Lucie Dehouck
(2)
,
Eloise Happernegg
(2)
,
Roland Bourette
(1)
,
Eric Adriaenssens
(1)
,
Chann Lagadec
(1)
,
Mehmet Tarhan
(7, 8, 9)
,
Dominique Collard
(10, 9)
,
Zied Souguir
(11)
,
Elodie Vandenhaute
(11)
,
Grégory Maubon
(11)
,
Nathalie Maubon
(11)
,
Francois Sipieter
(12)
,
François Sipieter
(12)
,
Nicolas Borghi
(12)
,
Paolo Giacobini
(4, 5)
,
Vincent Prevost
(5)
,
Fabien Gosselet
(2)
,
Xuefen Le Bourhis
(1)
,
Isabelle van Seuningen
(13, 1)
,
Caroline Mysiorek
(2)
,
Robert-Alain Toillon
(1)
1
CANTHER -
Cancer Heterogeneity, Plasticity and Resistance to Therapies - UMR 9020 - U 1277
2 LBHE - Laboratoire de la Barrière Hémato-Encéphalique
3 UNICANCER/Lille - Centre Régional de Lutte contre le Cancer Oscar Lambret [Lille]
4 CHRU Lille - Centre Hospitalier Régional Universitaire [CHU Lille]
5 LilNCog - Lille Neurosciences & Cognition - U 1172
6 PLBS - Plateformes Lilloises en Biologie et Santé - UAR 2014 - US 41
7 IEMN - Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520
8 BIOMEMS - IEMN - Bio-Micro-Electro-Mechanical Systems - IEMN
9 JUNIA - JUNIA
10 LIMMS - Laboratory for Integrated Micro Mechatronics Systems
11 HCS-PL - HCS-Pharma Loos [Lille]
12 IJM (UMR_7592) - Institut Jacques Monod
13 INSERM - Institut National de la Santé et de la Recherche Médicale
2 LBHE - Laboratoire de la Barrière Hémato-Encéphalique
3 UNICANCER/Lille - Centre Régional de Lutte contre le Cancer Oscar Lambret [Lille]
4 CHRU Lille - Centre Hospitalier Régional Universitaire [CHU Lille]
5 LilNCog - Lille Neurosciences & Cognition - U 1172
6 PLBS - Plateformes Lilloises en Biologie et Santé - UAR 2014 - US 41
7 IEMN - Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520
8 BIOMEMS - IEMN - Bio-Micro-Electro-Mechanical Systems - IEMN
9 JUNIA - JUNIA
10 LIMMS - Laboratory for Integrated Micro Mechatronics Systems
11 HCS-PL - HCS-Pharma Loos [Lille]
12 IJM (UMR_7592) - Institut Jacques Monod
13 INSERM - Institut National de la Santé et de la Recherche Médicale
Sarah Trouvilliez
- Fonction : co premier-auteur
Chann Lagadec
- Fonction : Auteur
- PersonId : 1066377
- IdHAL : chann-lagadec
Mehmet Tarhan
- Fonction : Auteur
- PersonId : 748912
- IdHAL : mehmet-cagatay-tarhan
Francois Sipieter
- Fonction : Auteur
- PersonId : 1165031
- IdHAL : francois-sipieter
- ORCID : 0000-0002-1332-400X
- IdRef : 192590766
Nicolas Borghi
- Fonction : Auteur
- PersonId : 738445
- IdHAL : nicolas-borghi
- ORCID : 0000-0002-1558-5246
- IdRef : 116205857
Fabien Gosselet
- Fonction : Auteur
- PersonId : 747473
- IdHAL : fabgoss
- ORCID : 0000-0002-0481-5026
- IdRef : 112931200
Xuefen Le Bourhis
- Fonction : Auteur
- PersonId : 1138707
- IdHAL : xuefen-le-bourhis
Isabelle van Seuningen
- Fonction : Auteur
- PersonId : 179339
- IdHAL : isabelle-van-seuningen
- ORCID : 0000-0002-3131-2694
- IdRef : 094282048
Caroline Mysiorek
- Fonction : co dernier-auteur
- PersonId : 1145912
- IdHAL : caroline-mysiorek
- ORCID : 0000-0003-2338-8821
- IdRef : 14658211X
Robert-Alain Toillon
Connectez-vous pour contacter l'auteur
- Fonction : Auteur correspondant
- PersonId : 740687
- IdHAL : robert-alain-toillon
- ORCID : 0000-0001-5483-2118
- IdRef : 059903732
Connectez-vous pour contacter l'auteur
Résumé
With nearly 2.3 million cases diagnosed worldwide each year and an estimated 685 000 deaths by 2020, breast cancer is the leading cause of cancer-related death in women. Brain metastases cause severe cognitive complications that severely impair quality of life. In TN breast cancer, the prognosis for brain metastases is particularly poor with a median survival of no more than 6 months. It is therefore crucial to know the molecular actors that promote the metastatic dissemination of triple negative breast cancer to the brain but also to prevent the proliferation of brain micrometastases that can cause fatal recurrences.
In order to recapitulate several final stages of brain metastasis, we used both human Blood- Brain-Barrier in vitro model, human organotypic 3D extracellular in vitro matrix, mice brain slices organotypic ex vivo culture and in vivo mice xenograft. These models are coupled with single cell, 3D and real time imaging techniques, including FRET biosensing. This unique experimental approach allows us to study the involvement of signaling pathways in these different biological processes. Using this innovative method, we have identified that inhibition neurotrophins receptor leads to a decrease in the activity of the underlying signaling pathways and consequently to a decrease in the ability of breast cancer cells to pass through the BBB, to grow and to colonize the brain parenchyma.
Domaines
Sciences de l'ingénieur [physics]Format du dépôt | Notice |
---|---|
Type de dépôt | Communication dans un congrès |
Titre |
en
Neurotrophins promotes brain metastasis of triple negative breast cancer through Src kinase family activation
|
Résumé |
en
With nearly 2.3 million cases diagnosed worldwide each year and an estimated 685 000 deaths by 2020, breast cancer is the leading cause of cancer-related death in women. Brain metastases cause severe cognitive complications that severely impair quality of life. In TN breast cancer, the prognosis for brain metastases is particularly poor with a median survival of no more than 6 months. It is therefore crucial to know the molecular actors that promote the metastatic dissemination of triple negative breast cancer to the brain but also to prevent the proliferation of brain micrometastases that can cause fatal recurrences.
In order to recapitulate several final stages of brain metastasis, we used both human Blood- Brain-Barrier in vitro model, human organotypic 3D extracellular in vitro matrix, mice brain slices organotypic ex vivo culture and in vivo mice xenograft. These models are coupled with single cell, 3D and real time imaging techniques, including FRET biosensing. This unique experimental approach allows us to study the involvement of signaling pathways in these different biological processes. Using this innovative method, we have identified that inhibition neurotrophins receptor leads to a decrease in the activity of the underlying signaling pathways and consequently to a decrease in the ability of breast cancer cells to pass through the BBB, to grow and to colonize the brain parenchyma.
|
Auteur(s) |
Julien Cicero
1, 2, 3
, Sarah Trouvilliez
1
, Martine Palma
1
, Gaetan Ternier
4, 5
, Laurine Decoster
4, 5
, Nicolas Barrois
6
, Lucie Dehouck
2
, Eloise Happernegg
2
, Roland Bourette
1
, Eric Adriaenssens
1
, Chann Lagadec
1
, Mehmet Tarhan
7, 8, 9
, Dominique Collard
10, 9
, Zied Souguir
11
, Elodie Vandenhaute
11
, Grégory Maubon
11
, Nathalie Maubon
11
, Francois Sipieter
12
, François Sipieter
12
, Nicolas Borghi
12
, Paolo Giacobini
4, 5
, Vincent Prevost
5
, Fabien Gosselet
2
, Xuefen Le Bourhis
1
, Isabelle van Seuningen
13, 1
, Caroline Mysiorek
2
, Robert-Alain Toillon
1
1
CANTHER -
Cancer Heterogeneity, Plasticity and Resistance to Therapies - UMR 9020 - U 1277
( 1004619 )
- Laboratoire CANTHER, UMR 9020 CNRS-1277 Inserm --
Hétérogénéité, Plasticité et Résistance aux Thérapies des Cancers -
Université de Lille - Bâtiment Cancer - Place de Verdun -
59000 LILLE
- France
2
LBHE -
Laboratoire de la Barrière Hémato-Encéphalique
( 1003553 )
- Faculté Jean Perrin, Rue Jean Souvraz, 62300 LENS
- France
3
UNICANCER/Lille -
Centre Régional de Lutte contre le Cancer Oscar Lambret [Lille]
( 301886 )
- 3, rue Frédéric Combemale, 59020 Lille
- France
4
CHRU Lille -
Centre Hospitalier Régional Universitaire [CHU Lille]
( 425779 )
- CHU/CHRU Lille - 2, avenue Oscar Lambret - 59037 Lille Cedex
- France
5
LilNCog -
Lille Neurosciences & Cognition - U 1172
( 1049721 )
- (ex-JPARC) --
Université de Lille
- Faculté de Médecine - Bâtiment Biserte
- 1 place de Verdun
- 59045 Lille Cedex
- Tél : +33 (0)3 20 29 88 50
- France
6
PLBS -
Plateformes Lilloises en Biologie et Santé - UAR 2014 - US 41
( 1040364 )
- Université de Lille - Cité Scientifique - Bâtiment C9 - Bureau 104 -
59655 Villeneuve d'ascq
- France
7
IEMN -
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520
( 1066983 )
- [Univ. Lille, CNRS, Centrale Lille Institut, Junia, Univ. Polytechnique Hauts-de-France] ––
Laboratoire Central – Cité Scientifique – Avenue Poincaré – CS 60069 – 59652 VILLENEUVE D’ASCQ CEDEX
- France
8
BIOMEMS - IEMN -
Bio-Micro-Electro-Mechanical Systems - IEMN
( 1067476 )
- Groupe BioMEMS - IEMN UMR8520
- France
9
JUNIA -
JUNIA
( 1059196 )
- 2 Rue Norbert Segard
59014 Lille
- France
10
LIMMS -
Laboratory for Integrated Micro Mechatronics Systems
( 2208 )
- LIMMS/CNRS-IIS(UMI 2820)/ Institute of Industrial Science, University of Tokyo/ 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505 Japan/
- Japon
11
HCS-PL -
HCS-Pharma Loos [Lille]
( 1083242 )
- Biocentre Fleming, 250 rue Salvador Allende, Bat A, 59120 Loos
- France
12
IJM (UMR_7592) -
Institut Jacques Monod
( 1004865 )
- Université Paris Cité, Bât. Buffon, 15 rue Hélène Brion, 75205 Paris cédex 13
- France
13
INSERM -
Institut National de la Santé et de la Recherche Médicale
( 303623 )
- 101, rue de Tolbiac, 75013 Paris
- France
|
Langue du document |
Anglais
|
Vulgarisation |
Non
|
Comité de lecture |
Non
|
Invité |
Non
|
Audience |
Internationale
|
Actes |
Non
|
Titre du congrès |
1st ONCOLille days 2022
|
Date début congrès |
2022-11-02
|
Date fin congrès |
2022-11-04
|
Ville |
Lille
|
Pays |
France
|
Commentaire |
ORAL
|
Domaine(s) |
|
Référence interne |
|
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