Cannabinoid during adolescence phenocopies reelin haploinsufficiency in prefrontal cortex synapses - Archive ouverte HAL
Pré-Publication, Document De Travail Année : 2022

Cannabinoid during adolescence phenocopies reelin haploinsufficiency in prefrontal cortex synapses

Gabriele Giua
Olivier Lassalle
Michelle Murphy
  • Fonction : Auteur
Jim Wager-Miller
Ken Mackie
Olivier Manzoni

Résumé

ABSTRACT In humans and rodents, the protracted development of the prefrontal cortex (PFC) throughout adolescence represents a time for marked vulnerability towards environmental adversities, such as stress or drug exposure. We previously showed that the extracellular matrix protein reelin is an instrumental synaptic modulator that shapes medial PFC’s (mPFC) circuitry during maturation and is a critical mediator of the vulnerability to environmental stress. Emerging evidence highlight the role of the endocannabinoid system in the postnatal maturation of the PFC and reelin deficiency influences behavioral abnormalities caused by heavy consumption of THC during adolescence. Could the reelin-dependent maturation of prefrontal networks may be vulnerable to cannabinoid exposure during adolescence? To explore this hypothesis, we studied the effects of a single in-vivo exposure to a synthetic cannabinoid on reelin expression and mPFC functions in adolescent male mice. The results show that a single cannabinoid exposure mimics reelin haploinsufficiency by decreasing prefrontal reelin expression in a layer-specific pattern without changing its transcriptional levels. Furthermore, this treatment impeded synaptic plasticity: adolescent cannabinoid lowered long-term potentiation to the magnitude observed in age-matched reelin haploinsufficient males. Quantitative PCR analysis showed that changes in the mRNA levels of NMDARs does not account for the reduction of TBS-LTP. Together, the data show that exposure to cannabinoid during adolescence phenocopies reelin haploinsufficiency and further identifies reelin as a key component of the vulnerability of PFC to environmental insults.

Dates et versions

hal-03852180 , version 1 (14-11-2022)

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T.J. Thenzing Juda Silva-Hurtado, Gabriele Giua, Olivier Lassalle, Michelle Murphy, Jim Wager-Miller, et al.. Cannabinoid during adolescence phenocopies reelin haploinsufficiency in prefrontal cortex synapses. 2022. ⟨hal-03852180⟩
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