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Article Dans Une Revue Nature Communications Année : 2022

On the fluorescence enhancement of arch neuronal optogenetic reporters

Résumé

The lack of a theory capable of connecting the amino acid sequence of a lightabsorbing protein with its fluorescence brightness is hampering the development of tools for understanding neuronal communications. Here we demonstrate that a theory can be established by constructing quantum chemical models of a set of Archaerhodopsin reporters in their electronically excited state. We found that the experimentally observed increase in fluorescence quantum yield is proportional to the computed decrease in energy difference between the fluorescent state and a nearby photoisomerization channel leading to an exotic diradical of the protein chromophore. This finding will ultimately support the development of technologies for searching novel fluorescent rhodopsin variants and unveil electrostatic changes that make light emission brighter and brighter.

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Chimie
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hal-03846130 , version 1 (10-11-2022)

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Leonardo Barneschi, Emanuele Marsili, Laura Pedraza-González, Daniele Padula, Luca de Vico, et al.. On the fluorescence enhancement of arch neuronal optogenetic reporters. Nature Communications, 2022, 13 (1), pp.6432. ⟨10.1038/s41467-022-33993-4⟩. ⟨hal-03846130⟩
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