Control of the proportion of inner cells by asymmetric divisions and the ensuing resilience of cloned rabbit embryos - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Development (Cambridge, England) Année : 2018

Control of the proportion of inner cells by asymmetric divisions and the ensuing resilience of cloned rabbit embryos

Résumé

Mammalian embryo cloning by nuclear transfer has a low success rate. This is hypothesized to correlate with a high variability of early developmental steps that segregate outer cells, which are fated to extra-embryonic tissues, from inner cells, which give rise to the embryo proper. Exploring the cell lineage of wild-type embryos and clones, imaged in toto until hatching, highlights the respective contributions of cell proliferation, death and asymmetric divisions to phenotypic variability. Preferential cell death of inner cells in clones, probably pertaining to the epigenetic plasticity of the transferred nucleus, is identified as a major difference with effects on the proportion of inner cell. In wild type and clones, similar patterns of outer cell asymmetric divisions are shown to be essential to the robust proportion of inner cells observed in wild type. Asymmetric inner cell division, which is not described in mice, is identified as a regulator of the proportion of inner cells and likely gives rise to resilient clones.
Fichier principal
Vignette du fichier
2018_Fabreges_Development_1.pdf (2.64 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-01831822 , version 1 (26-05-2020)

Licence

Paternité

Identifiants

Citer

Dimitri Fabrèges, Nathalie Daniel, Véronique Duranthon, Nadine Peyriéras. Control of the proportion of inner cells by asymmetric divisions and the ensuing resilience of cloned rabbit embryos. Development (Cambridge, England), 2018, 145 (8), pp.1-7. ⟨10.1242/dev.152041⟩. ⟨hal-01831822⟩
123 Consultations
26 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More