Recruitment of Activated G Protein-coupled Receptors to Pre-existing Clathrin-coated Pits in Living Cells - Archive ouverte HAL
Article Dans Une Revue Journal of Biological Chemistry Année : 2002

Recruitment of Activated G Protein-coupled Receptors to Pre-existing Clathrin-coated Pits in Living Cells

Résumé

The process of clathrin-mediated endocytosis tightly regulates signaling of the superfamily of seven-transmembrane G protein-coupled receptors (GPCRs). A fundamental question in the cell biology of membrane receptor endocytosis is whether activated receptors can initiate the formation of clathrin-coated pits (CPs) or whether they are simply mobilized to pre-existing CPs. Here, using various approaches, including a dynamic assay to monitor the distribution of CPs and GPCR-beta-arrestin complexes in live HeLa cells, we demonstrate for the first time that activated GPCRs do not initiate the de novo formation of CPs but instead are targeted to pre-existing CPs.

Dates et versions

hal-03844234 , version 1 (08-11-2022)

Identifiants

Citer

Mark G.H. Scott, Alexandre Benmerah, Olivier Muntaner, Stefano Marullo. Recruitment of Activated G Protein-coupled Receptors to Pre-existing Clathrin-coated Pits in Living Cells. Journal of Biological Chemistry, 2002, 277 (5), pp.3552-3559. ⟨10.1074/jbc.M106586200⟩. ⟨hal-03844234⟩
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