Blocking the IL-23/Th17 pathway ameliorates myasthenia gravis symptoms in autoimmune myasthenia gravis mouse model - Archive ouverte HAL Accéder directement au contenu
Communication Dans Un Congrès Année : 2021

Blocking the IL-23/Th17 pathway ameliorates myasthenia gravis symptoms in autoimmune myasthenia gravis mouse model

Résumé

Background and aims Autoimmune myasthenia gravis (MG) is caused by autoantibodies directed against the acetylcholine receptor AChR). In MG, the thymus is the effector tissue characterized by a chronic inflammation, defective regulatory T-cells and ectopic germinal centers (eGCs) (Berrih-Aknin 2014). A crosstalk between Th17 cells and thymic epithelial cells, mediated by IL-23 and IL-17, sustains thymic events supporting antibody production by B-cells found in eGCs (Villegas et al. 2019). We analyzed the effect of blocking the IL-23/Th17 axis, on the thymus and MG clinical course in a MG preclinical mouse model. Methods Immune-deficient NSG mice were engrafted with AChR+ MG thymic biopsies (NSG-MG). The NSG-MG model recapitulates MG symptoms and development (Sudres et al. 2017). Twenty five days after engraftment, mice were treated with a monoclonal anti human IL-23 antibody. We assessed the treatment effects on the engrafted thymic biopsies, muscle homeostasis and von MG global clinical score. Results The treatment limited or stopped the activation of inflammatory T-cells (CD4+CCR6+ CCR4+ T- cells) in the thymus and the periphery. In addition, a decreased thymic expression of protein involved in eGC stabilization was observed. More, increased muscle regeneration gene expression and significant muscular improvements were observed and correlated with amelioration of clinical symptoms and global disease score. Conclusion Altogether these data suggest that an anti-IL-23 therapy could be beneficial in MG by acting on the thymic inflammatory state and on muscle physiology. The inhibition of IL-23 activates transduction pathways involved in antibody production and in muscle regeneration process favoring then disease amelioration.
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Dates et versions

hal-03844107 , version 1 (15-11-2022)

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  • HAL Id : hal-03844107 , version 1

Citer

Jose Adolfo Villegas, Sonia Berrih-Aknin, Rozen Le Panse, Nadine Dragin. Blocking the IL-23/Th17 pathway ameliorates myasthenia gravis symptoms in autoimmune myasthenia gravis mouse model. 12th international congress on autoimmunity, May 2021, virtual, France. ⟨hal-03844107⟩
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