The Telesphore project aims to identify and to quantify the transfers of pharmaceuticals residues and biocides and their related processes from biosolids and manure spread on grassland as fertilizers. Real land-spreading conditions were reproduced on six in-situ lysimeters and the pathway of pharmaceuticals and biocides was tracked by sampling infiltrated water and soil over one year. The study has focused on 32 pharmaceuticals, biocides and their transformation products, selected from their previous detection in several organic fertilizers. Six months after biosolids and manure spreading, the soil concentrations were in the range of nanograms per gram of soil and only few compounds were detected in infiltrated water. Ketoprofen was found to be the most mobile and persistent pharmaceutical compound after biosolids application whereas thiabendazole was the most frequently detected substance in infiltrated water after manure amendment.