Selective Plane Illumination Microscope dedicated to volumetric imaging in microfluidic chambers
Résumé
In this article, we are presenting an original selective plane illumination fluorescence microscope dedicated to image Organ-on-chip-like biostructures in microfluidic chips. In order to be able to morphologically analyze volumetric samples in development at the cellular scale inside microfluidic chambers, the setup presents a compromise between relatively large field of view (~ 200 μm) and moderate resolution (~ 5 μm). The microscope is based on a simple design, built around the chip and its microfluidic environment to allow 3D imaging inside the chip. In particular, the sample remains horizontally avoiding to disturb the fluidics phenomena. The experimental setup, its optical characterization and the first volumetric images are reported.
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