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Article Dans Une Revue Scientific Reports Année : 2022

Reconstruction of functional human epidermis equivalent containing 5%IPS-derived keratinocytes treated with mitochondrial stimulating plant extracts

Marielle Moreau
  • Fonction : Auteur
Christophe Capallere
  • Fonction : Auteur
Christelle Plaza
  • Fonction : Auteur
Céline Meyrignac
  • Fonction : Auteur
Karl Pays
  • Fonction : Auteur
Bruno Bavouzet
  • Fonction : Auteur
Jean-Marie Botto
  • Fonction : Auteur
Carine Nizard
  • Fonction : Auteur
Anne-Laure Bulteau

Résumé

Abstract Reconstructed human epidermis equivalents (RHE) have been developed as a clinical skin substitute and as the replacement for animal testing in both research and industry. KiPS, or keratinocytes derived from induced pluripotent stem cells (iPSCs) are frequently used to generate RHE. In this study, we focus on the mitochondrial performance of the KiPS derived from iPSCs obtained from two donors. We found that the KiPS derived from the older donor have more defective mitochondria. Treatment of these KiPS with a plant extract enriched in compounds known to protect mitochondria improved mitochondrial respiration and rendered them fully competent to derive high-quality RHE. Overall, our results suggest that improving mitochondrial function in KiPS is one of the key aspects to obtain a functional RHE and that our plant extracts can improve in this process.
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Dates et versions

hal-03830642 , version 1 (10-11-2022)

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Marielle Moreau, Christophe Capallere, Laurent Chavatte, Christelle Plaza, Céline Meyrignac, et al.. Reconstruction of functional human epidermis equivalent containing 5%IPS-derived keratinocytes treated with mitochondrial stimulating plant extracts. Scientific Reports, 2022, 12 (1), pp.9073. ⟨10.1038/s41598-022-13191-4⟩. ⟨hal-03830642⟩
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