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                <term xml:lang="en">NFL-TBS.40-63 peptide</term>
                <term xml:lang="en">the scrambled NFL-TBS.40–63 peptide (with the same amino acids as the NFL-peptide but in a different order)</term>
                <term xml:lang="en">NFL-peptide</term>
                <term xml:lang="en">neurofilament light subunit derived 24 amino acid tubulin-binding site peptide NFL-TBS.40–63</term>
                <term xml:lang="en">SDS</term>
                <term xml:lang="en">sodium dodecyl sulfate</term>
                <term xml:lang="en">Self-assembly</term>
                <term xml:lang="en">TEM</term>
                <term xml:lang="en">transmission electron microscopy</term>
                <term xml:lang="en">Targeted drug delivery</term>
                <term xml:lang="en">UPLC</term>
                <term xml:lang="en">ultra-performance liquid chromatography system</term>
                <term xml:lang="en">XRS</term>
                <term xml:lang="en">CAC</term>
                <term xml:lang="en">x-ray scattering</term>
                <term xml:lang="en">aCSF</term>
                <term xml:lang="en">artificial cerebrospinal fluid</term>
                <term xml:lang="en">nude-NFL</term>
                <term xml:lang="en">the NFL-TBS.40–63 peptide with no labeling</term>
                <term xml:lang="en">BIOT-NFL</term>
                <term xml:lang="en">the NFL-TBS.40–63 peptide coupled to the biotin</term>
                <term xml:lang="en">BIOT-NFL-peptide-nanofibers</term>
                <term xml:lang="en">critical aggregation concentration</term>
                <term xml:lang="en">DMEM</term>
                <term xml:lang="en">dulbecco's modified eagle medium</term>
                <term xml:lang="en">FAM-NFL</term>
                <term xml:lang="en">the NFL-TBS.40–63 peptide coupled to 5-carboxy-fluorescein</term>
                <term xml:lang="en">Glioblastoma</term>
                <term xml:lang="en">Gold nanoparticles</term>
                <term xml:lang="en">NFL-SCR</term>
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              <p>NFL-TBS.40-63 peptide is a recently discovered peptide derived from the light neurofilament chain (NFL). In this study, we demonstrated that the Biotinylated-NFL-peptide (BIOT-NFL) can spontaneously self-assemble into well-organized nanofibers (approximately 5 nm width and several micrometers in length) in several solutions, whereas the typical self-assembly was not systematically observed from other peptides with or without coupling. The critical aggregation concentration that allows the BIOT-NFL-peptide to aggregate and auto associate was determined at 10-4 mol/L by surface tension measurements. X-ray scattering of BIOT-NFL-peptide also demonstrated its beta-sheet structure that can facilitate the intermolecular interactions involved in the self-assembly process. The possible disassembly of self-assembled BIOT-NFL-peptide-nanofibers was examined via a dialysis membrane study. We further investigated the interaction between nanofibers formed by BIOT-NFL-peptide and gold nanoparticles. Interestingly, a strong interaction was demonstrated between these nanoparticles and BIOT-NFL-peptide resulted in the formation of BIOT-NFL-peptide-nanofibers grandly decorated by gold nanoparticles. Finally, we investigated the internalization of gold nanoparticles coupled with BIOT-NFL-nanofibers into F98 rat glioblastoma cells, which was increased compared to the non-coupled control gold nanoparticles. All these results indicate that this peptide could be a promising therapeutic agent for targeted delivery.</p>
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