Direct measurement of the aerotactic response in a bacterial suspension - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Physical Review E Année : 2022

Direct measurement of the aerotactic response in a bacterial suspension

Résumé

Aerotaxis is the ability of motile cells to navigate toward oxygen. A key question is the dependence of the aerotactic velocity with the local oxygen concentration c. Here we combine simultaneous bacteria tracking and local oxygen concentration measurements using Ruthenium encapsulated in micelles to characterize the aerotactic response of Burkholderia contaminans, a motile bacterium ubiquitous in the environment. In our experiments, an oxygen gradient is produced by the bacterial respiration in a sealed glass capillary permeable to oxygen at one end, producing a bacterial band traveling toward the oxygen source. We compute the aerotactic response χ(c) both at the population scale, from the drift velocity in the bacterial band, and at the bacterial scale, from the angular modulation of the run times. Both methods are consistent with a power-law χ ∝ c−2, in good agreement with existing models based on the biochemistry of bacterial membrane receptors.
Fichier principal
Vignette du fichier
2203.04656 (9.77 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-03823291 , version 1 (19-12-2023)

Identifiants

Citer

J. Bouvard, Carine Douarche, P. Mergaert, H. Auradou, Frederic Moisy. Direct measurement of the aerotactic response in a bacterial suspension. Physical Review E , 2022, 106 (3), pp.034404. ⟨10.1103/PhysRevE.106.034404⟩. ⟨hal-03823291⟩
33 Consultations
4 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More