Discovery of Small-Molecule Inhibitors of the PTK7/β-Catenin Interaction Targeting the Wnt Signaling Pathway in Colorectal Cancer - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue ACS Chemical Biology Année : 2022

Discovery of Small-Molecule Inhibitors of the PTK7/β-Catenin Interaction Targeting the Wnt Signaling Pathway in Colorectal Cancer

Résumé

Second cause of death due to cancer worldwide, colorectal cancer (CRC) is a major public health issue. The discovery of new therapeutic targets is thus essential. The pseudokinase PTK7 intervenes in the regulation of the Wnt/βcatenin pathway signaling, in part, through a kinase-domain dependent interaction with the β-catenin protein. PTK7 is overexpressed in CRC; an event associated with metastatic development and reduced survival of non-metastatic patient. In addition, numerous alterations have been identified in CRC inducing constitutive activation of Wnt/β-catenin pathway signaling through β-catenin accumulation. Thus,targeting PTK7/β-catenin interaction could be of interest for future drug development. We have developed a NanoBRET TM screening assay recapitulating the interaction between PTK7 and βcatenin to identify compounds able to disrupt this protein-protein interaction. A high-throughput screening allowed us to identify small molecule inhibitors targeting the Wnt pathway signaling and inducing anti-proliferative and anti-tumor effect in vitro in CRC cells harboring β-catenin or APC mutations. Thus, inhibition of the PTK7/β-catenin interaction could represent a new therapeutic strategy to inhibit cell growth dependent on Wnt signaling pathway. Moreover, despite a lack of enzymatic activity of its tyrosine kinase domain, targeting the PTK7 kinase domain-dependent functions appears to be of interest for further therapeutic development.
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Dates et versions

hal-03817750 , version 1 (17-10-2022)

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Laetitia Ganier, Stéphane Betzi, Carine Derviaux, Philippe Roche, Charlotte Dessaux, et al.. Discovery of Small-Molecule Inhibitors of the PTK7/β-Catenin Interaction Targeting the Wnt Signaling Pathway in Colorectal Cancer. ACS Chemical Biology, 2022, 17 (5), pp.1061-1072. ⟨10.1021/acschembio.1c00826⟩. ⟨hal-03817750⟩
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