Wnt/(beta)-catenin signaling regulates the expression of the homeobox gene Cdx1 in embryonic intestine - Archive ouverte HAL
Article Dans Une Revue Development (Cambridge, England) Année : 2000

Wnt/(beta)-catenin signaling regulates the expression of the homeobox gene Cdx1 in embryonic intestine

H. Lickert
  • Fonction : Auteur
Claire Domon
  • Fonction : Auteur
  • PersonId : 1101801
G. Huls
  • Fonction : Auteur
C. Wehrle
  • Fonction : Auteur
Isabelle Duluc
  • Fonction : Auteur
  • PersonId : 1119751
H. Clevers
  • Fonction : Auteur
B. I. Meyer
  • Fonction : Auteur
Jean-Noël Freund
R. Kemler
  • Fonction : Auteur

Résumé

During mammalian development, the Cdx1 homeobox gene exhibits an early period of expression when the embryonic body axis is established, and a later period where expression is restricted to the embryonic intestinal endoderm. Cdx1 expression is maintained throughout adulthood in the proliferative cell compartment of the continuously renewed intestinal epithelium, the crypts. In this study, we provide evidence in vitro and in vivo that Cdx1 is a direct transcriptional target of the Wnt/(beta)-catenin signaling pathway. Upon Wnt stimulation, expression of Cdx1 can be induced in mouse embryonic stem (ES) cells as well as in undifferentiated rat embryonic endoderm. Tcf4-deficient mouse embryos show abrogation of Cdx1 protein in the small intestinal epithelium, making Tcf4 the likely candidate to transduce Wnt signal in this part of gut. The promoter region of the Cdx1 gene contains several Tcf-binding motifs, and these bind Tcf/Lef1/(beta)-catenin complexes and mediate (beta)-catenin-dependent transactivation. The transcriptional regulation of the homeobox gene Cdx1 in the intestinal epithelium by Wnt/(beta)-catenin signaling underlines the importance of this signaling pathway in mammalian endoderm development.
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Dates et versions

hal-03812792 , version 1 (12-10-2022)

Identifiants

  • HAL Id : hal-03812792 , version 1

Citer

H. Lickert, Claire Domon, G. Huls, C. Wehrle, Isabelle Duluc, et al.. Wnt/(beta)-catenin signaling regulates the expression of the homeobox gene Cdx1 in embryonic intestine. Development (Cambridge, England), 2000, 127 (17), pp.3805-3813. ⟨hal-03812792⟩
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