Enteropathogenic e.coli sustains iodoacetamide-induced ulcerative colitis-like colitis in rats: modulation of IL-1β, IL-6, TNF-α, COX-2, and apoptosisi - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Biological Regulators and Homeostatic Agents Année : 2012

Enteropathogenic e.coli sustains iodoacetamide-induced ulcerative colitis-like colitis in rats: modulation of IL-1β, IL-6, TNF-α, COX-2, and apoptosisi

I. Abdallah Hajj Hussein
  • Fonction : Auteur
Jean-Marie Reimund
  • Fonction : Auteur
  • PersonId : 1129935
A. Shams
  • Fonction : Auteur
M. Yamine
  • Fonction : Auteur
A. Leone
  • Fonction : Auteur
A. R. Jurjus
  • Fonction : Auteur

Résumé

Pathogenic or non-pathogenic bacteria from flora may play a key role in inflammatory bowel disease (IBD) pathogenesis. However, a specific infectious agent causing IBD has not been identified. This study assessed the impact of enteropathogenic E. coli (EPEC) on the modulation of IL-1beta, IL-6, TNF- alpha, COX-2, BAX and Bcl-2 expression, in sustaining inflammation of a rat colitis model. Two hundred male Sprague-Dawley rats (4 groups) were inoculated weekly or bi-weekly for 70 days, with 1 percent methylcellulose (MC), (b) 6 percent iodoacetamide (IA) in 1 percent MC, (c) 4x108 CFU of EPEC, and (d) IA+EPEC. After a month, treatment was stopped in half of the animals in each group. IL-1beta, IL-6, TNF-alpha, COX-2, BAX and Bcl-2 expression were measured in colonic mucosa scrapings. IL-1beta, IL-6, TNF-alpha, and COX-2 were significantly increased in colonic mucosa of the IA+EPEC group and to a lesser but significant level in the IA group compared to controls, or EPEC alone, both in continued and discontinued treatment groups. Additionally, the BAX/Bcl-2 ratio decreased, indicating less apoptosis in the IA+EPEC group which exhibited more necrosis. These effects increased with experiment duration. This work provides new arguments favouring the role of bacteria in IBD pathogenesis.
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Dates et versions

hal-03812791 , version 1 (12-10-2022)

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  • HAL Id : hal-03812791 , version 1

Citer

I. Abdallah Hajj Hussein, Jean-Noël Freund, Jean-Marie Reimund, A. Shams, M. Yamine, et al.. Enteropathogenic e.coli sustains iodoacetamide-induced ulcerative colitis-like colitis in rats: modulation of IL-1β, IL-6, TNF-α, COX-2, and apoptosisi. Journal of Biological Regulators and Homeostatic Agents, 2012, 26 (3), pp.515-526. ⟨hal-03812791⟩
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