Article Dans Une Revue Biomaterials Année : 2019

Functionalized Polymer Microbubbles as New Molecular Ultrasound Contrast Agent to Target P-selectin in Thrombus

Résumé

Thrombotic diseases rarely cause symptoms until advanced stage and sudden death. Thus, early detection of thrombus by a widely spread imaging modality can improve the prognosis and reduce mortality. Here, polymer microbubbles (MBs) made of degradable poly(IsoButylCyanoAcrylate) and functionalized with fucoidan (Fucoidan-MBs) were designed as a new targeted ultrasound contrast agent to image venous thrombus. The physicochemical characterizations demonstrate that the MBs with fucoidan surface exhibit a size of 2–6 μm and stability in suspension at 4 °C up to 2 months. MBs exhibit high echogenicity and could be completely burst under high destructive pulse. Flow chamber experiments on activated human platelets show a higher affinity of Fucoidan-MBs than control anionic MBs (CM-Dextran-MBs) under shear stress conditions. In vivo analysis by ultrasound and histological results demonstrate that Fucoidan-MBs are localized in rat venous thrombotic wall, whereas few CM-Dextran-MBs are present. In addition, the binding of Fucoidan-MBs in healthy vein is not observed. Collectively, Fucoidan-MBs appear as a promising functionalized carrier for ultrasound molecular imaging in thrombotic diseases.

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hal-02387189 , version 1 (29-11-2019)

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Bo Li, Rachida Aid-Launais, Marie-Noëlle Labour, Alina Zenych, Maya Juenet, et al.. Functionalized Polymer Microbubbles as New Molecular Ultrasound Contrast Agent to Target P-selectin in Thrombus. Biomaterials, 2019, 194, pp.139-150. ⟨10.1016/j.biomaterials.2018.12.023⟩. ⟨hal-02387189⟩
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