<?xml version="1.0" encoding="utf-8"?>
<TEI xmlns="http://www.tei-c.org/ns/1.0" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:hal="http://hal.archives-ouvertes.fr/" xmlns:gml="http://www.opengis.net/gml/3.3/" xmlns:gmlce="http://www.opengis.net/gml/3.3/ce" version="1.1" xsi:schemaLocation="http://www.tei-c.org/ns/1.0 http://api.archives-ouvertes.fr/documents/aofr-sword.xsd">
  <teiHeader>
    <fileDesc>
      <titleStmt>
        <title>HAL TEI export of hal-03798229</title>
      </titleStmt>
      <publicationStmt>
        <distributor>CCSD</distributor>
        <availability status="restricted">
          <licence target="https://creativecommons.org/publicdomain/zero/1.0/">CC0 1.0 - Universal</licence>
        </availability>
        <date when="2026-05-17T06:54:57+02:00"/>
      </publicationStmt>
      <sourceDesc>
        <p part="N">HAL API Platform</p>
      </sourceDesc>
    </fileDesc>
  </teiHeader>
  <text>
    <body>
      <listBibl>
        <biblFull>
          <titleStmt>
            <title xml:lang="en">Visualizing Mitochondrial Importability of a Protein Using the Yeast Bi-Genomic Mitochondrial-Split-GFP Strain and an Ordinary Fluorescence Microscope</title>
            <author role="aut">
              <persName>
                <forename type="first">Marine</forename>
                <surname>Hemmerle</surname>
              </persName>
              <email type="md5">4a57d014941a479fbcc2e43190493e44</email>
              <email type="domain">unistra.fr</email>
              <idno type="idhal" notation="numeric">1075859</idno>
              <idno type="halauthorid" notation="string">2000609-1075859</idno>
              <idno type="IDREF">https://www.idref.fr/260882135</idno>
              <affiliation ref="#struct-93783"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Bruno</forename>
                <surname>Senger</surname>
              </persName>
              <email type="md5">1e83ec513e844e7bd8f72ad2e63de8e2</email>
              <email type="domain">unistra.fr</email>
              <idno type="idhal" notation="string">bruno-senger</idno>
              <idno type="idhal" notation="numeric">738414</idno>
              <idno type="halauthorid" notation="string">50793-738414</idno>
              <idno type="ORCID">https://orcid.org/0000-0002-5157-7307</idno>
              <idno type="IDREF">https://www.idref.fr/18989752X</idno>
              <affiliation ref="#struct-93783"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Roza</forename>
                <surname>Kucharczyk</surname>
              </persName>
              <idno type="halauthorid">661104-0</idno>
              <affiliation ref="#struct-303306"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Hubert</forename>
                <surname>Becker</surname>
              </persName>
              <email type="md5">bc6a04aabfbe086753906a433bc359f1</email>
              <email type="domain">ibmc.u-strasbg.fr</email>
              <idno type="idhal" notation="numeric">1073400</idno>
              <idno type="halauthorid" notation="string">375971-1073400</idno>
              <idno type="ORCID">https://orcid.org/0000-0002-4102-7520</idno>
              <idno type="IDREF">https://www.idref.fr/151566704</idno>
              <idno type="ORCID">https://orcid.org/0000-0003-3136-6075</idno>
              <idno type="VIAF">https://viaf.org/viaf/194133247</idno>
              <idno type="RESEARCHERID">http://www.researcherid.com/rid/CEO-2039-2022</idno>
              <idno type="RESEARCHERID">http://www.researcherid.com/rid/HRV-7236-2023</idno>
              <idno type="RESEARCHERID">http://www.researcherid.com/rid/http://www.researcherid.com/rid/CEO-2039-2022</idno>
              <idno type="RESEARCHERID">http://www.researcherid.com/rid/http://www.researcherid.com/rid/HRV-7236-2023</idno>
              <affiliation ref="#struct-93783"/>
            </author>
            <editor role="depositor">
              <persName>
                <forename>Bruno</forename>
                <surname>Senger</surname>
              </persName>
              <email type="md5">1e83ec513e844e7bd8f72ad2e63de8e2</email>
              <email type="domain">unistra.fr</email>
            </editor>
          </titleStmt>
          <editionStmt>
            <edition n="v1" type="current">
              <date type="whenSubmitted">2022-10-05 10:45:07</date>
              <date type="whenModified">2025-09-09 09:56:27</date>
              <date type="whenReleased">2022-10-06 14:23:05</date>
              <date type="whenProduced">2022-07-01</date>
              <date type="whenEndEmbargoed">2022-10-05</date>
              <ref type="file" target="https://hal.science/hal-03798229v1/document">
                <date notBefore="2022-10-05"/>
              </ref>
              <ref type="file" subtype="author" n="1" target="https://hal.science/hal-03798229v1/file/Hemmerle%20MMB%202021full.pdf" id="file-3798250-3324152">
                <date notBefore="2022-10-05"/>
              </ref>
            </edition>
            <respStmt>
              <resp>contributor</resp>
              <name key="638382">
                <persName>
                  <forename>Bruno</forename>
                  <surname>Senger</surname>
                </persName>
                <email type="md5">1e83ec513e844e7bd8f72ad2e63de8e2</email>
                <email type="domain">unistra.fr</email>
              </name>
            </respStmt>
          </editionStmt>
          <publicationStmt>
            <distributor>CCSD</distributor>
            <idno type="halId">hal-03798229</idno>
            <idno type="halUri">https://hal.science/hal-03798229</idno>
            <idno type="halBibtex">hemmerle:hal-03798229</idno>
            <idno type="halRefHtml">&lt;i&gt;Mitochondria&lt;/i&gt;, 2497, Springer US, pp.255-267, 2022, Methods in Molecular Biology, &lt;a target="_blank" href="https://dx.doi.org/10.1007/978-1-0716-2309-1_16"&gt;&amp;#x27E8;10.1007/978-1-0716-2309-1_16&amp;#x27E9;&lt;/a&gt;</idno>
            <idno type="halRef">Mitochondria, 2497, Springer US, pp.255-267, 2022, Methods in Molecular Biology, &amp;#x27E8;10.1007/978-1-0716-2309-1_16&amp;#x27E9;</idno>
            <availability status="restricted">
              <licence target="https://about.hal.science/hal-authorisation-v1/">HAL Authorization<ref corresp="#file-3798250-3324152"/></licence>
            </availability>
          </publicationStmt>
          <seriesStmt>
            <idno type="stamp" n="INSERM">INSERM - Institut national de la santé et de la recherche médicale</idno>
            <idno type="stamp" n="CNRS">CNRS - Centre national de la recherche scientifique</idno>
            <idno type="stamp" n="UNIV-STRASBG">Université de Strasbourg</idno>
            <idno type="stamp" n="SITE-ALSACE">Archive ouverte du site Alsace</idno>
            <idno type="stamp" n="UNIVOAK">UNIVOAK</idno>
            <idno type="stamp" n="GMGM">Génétique moléculaire, génomique, microbiologie</idno>
          </seriesStmt>
          <notesStmt>
            <note type="audience" n="2">International</note>
            <note type="popular" n="0">No</note>
          </notesStmt>
          <sourceDesc>
            <biblStruct>
              <analytic>
                <title xml:lang="en">Visualizing Mitochondrial Importability of a Protein Using the Yeast Bi-Genomic Mitochondrial-Split-GFP Strain and an Ordinary Fluorescence Microscope</title>
                <author role="aut">
                  <persName>
                    <forename type="first">Marine</forename>
                    <surname>Hemmerle</surname>
                  </persName>
                  <email type="md5">4a57d014941a479fbcc2e43190493e44</email>
                  <email type="domain">unistra.fr</email>
                  <idno type="idhal" notation="numeric">1075859</idno>
                  <idno type="halauthorid" notation="string">2000609-1075859</idno>
                  <idno type="IDREF">https://www.idref.fr/260882135</idno>
                  <affiliation ref="#struct-93783"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Bruno</forename>
                    <surname>Senger</surname>
                  </persName>
                  <email type="md5">1e83ec513e844e7bd8f72ad2e63de8e2</email>
                  <email type="domain">unistra.fr</email>
                  <idno type="idhal" notation="string">bruno-senger</idno>
                  <idno type="idhal" notation="numeric">738414</idno>
                  <idno type="halauthorid" notation="string">50793-738414</idno>
                  <idno type="ORCID">https://orcid.org/0000-0002-5157-7307</idno>
                  <idno type="IDREF">https://www.idref.fr/18989752X</idno>
                  <affiliation ref="#struct-93783"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Roza</forename>
                    <surname>Kucharczyk</surname>
                  </persName>
                  <idno type="halauthorid">661104-0</idno>
                  <affiliation ref="#struct-303306"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Hubert</forename>
                    <surname>Becker</surname>
                  </persName>
                  <email type="md5">bc6a04aabfbe086753906a433bc359f1</email>
                  <email type="domain">ibmc.u-strasbg.fr</email>
                  <idno type="idhal" notation="numeric">1073400</idno>
                  <idno type="halauthorid" notation="string">375971-1073400</idno>
                  <idno type="ORCID">https://orcid.org/0000-0002-4102-7520</idno>
                  <idno type="IDREF">https://www.idref.fr/151566704</idno>
                  <idno type="ORCID">https://orcid.org/0000-0003-3136-6075</idno>
                  <idno type="VIAF">https://viaf.org/viaf/194133247</idno>
                  <idno type="RESEARCHERID">http://www.researcherid.com/rid/CEO-2039-2022</idno>
                  <idno type="RESEARCHERID">http://www.researcherid.com/rid/HRV-7236-2023</idno>
                  <idno type="RESEARCHERID">http://www.researcherid.com/rid/http://www.researcherid.com/rid/CEO-2039-2022</idno>
                  <idno type="RESEARCHERID">http://www.researcherid.com/rid/http://www.researcherid.com/rid/HRV-7236-2023</idno>
                  <affiliation ref="#struct-93783"/>
                </author>
              </analytic>
              <monogr>
                <title level="m">Mitochondria</title>
                <imprint>
                  <publisher>Springer US</publisher>
                  <pubPlace>New York, NY</pubPlace>
                  <biblScope unit="serie">Methods in Molecular Biology</biblScope>
                  <biblScope unit="volume">2497</biblScope>
                  <biblScope unit="pp">255-267</biblScope>
                  <date type="datePub">2022-07-01</date>
                </imprint>
              </monogr>
              <idno type="doi">10.1007/978-1-0716-2309-1_16</idno>
              <idno type="pubmed">35771447</idno>
            </biblStruct>
          </sourceDesc>
          <profileDesc>
            <langUsage>
              <language ident="en">English</language>
            </langUsage>
            <textClass>
              <keywords scheme="author">
                <term xml:lang="en">Saccharomyces cerevisiae</term>
                <term xml:lang="en">Mitochondria</term>
                <term xml:lang="en">Localization</term>
                <term xml:lang="en">Living cells</term>
                <term xml:lang="en">Epifluorescence microscopy</term>
                <term xml:lang="en">Dual localized</term>
                <term xml:lang="en">BiG Mito-Split-GFP</term>
              </keywords>
              <classCode scheme="mesh">Genomics</classCode>
              <classCode scheme="mesh">Green Fluorescent Proteins</classCode>
              <classCode scheme="mesh">Microscopy, Fluorescence</classCode>
              <classCode scheme="mesh">Mitochondria</classCode>
              <classCode scheme="mesh">Saccharomyces cerevisiae</classCode>
              <classCode scheme="halDomain" n="sdv">Life Sciences [q-bio]</classCode>
              <classCode scheme="halTypology" n="COUV">Book sections</classCode>
              <classCode scheme="halOldTypology" n="COUV">Book sections</classCode>
              <classCode scheme="halTreeTypology" n="COUV">Book sections</classCode>
            </textClass>
            <abstract xml:lang="en">
              <p>Proving with certainty that a GFP-tagged protein is imported inside mitochondria by visualizing its fluorescence emission with an epifluorescence microscope is currently impossible using regular GFP-tagging. This is particularly true for proteins dual localized in the cytosol and mitochondria, which have been estimated to represent up to one third of the established mitoproteomes. These proteins are usually composed of a surpassingly abundant pool of the cytosolic isoform compared to the mitochondrial isoform. As a consequence, when tagged with a regular GFP, the fluorescence emission of the cytosolic isoform will inevitably eclipse that of the mitochondrial one and prevent the detection of the mitochondrial echoform. To overcome this technical limit, we engineered a yeast strain expressing a new type of GFP called Bi-Genomic Mitochondrial-Split-GFP (BiG Mito-Split-GFP). In this strain, one moiety of the GFP is encoded by the mitochondrial DNA while the second moiety of the GFP can be tagged to any nuclear-encoded protein (suspected to be dual localized or bona fide mitochondrial). By doing so, only mitochondrial proteins or echoforms of dual localized proteins, regardless of their organismal origin, trigger GFP reconstitution that can be visualized by regular fluorescence microscopy. The strength of the BiG Mito-Split-GFP system is that proof of the mitochondrial localization of a given protein rests on a simple and effortless microscopy observation.</p>
            </abstract>
          </profileDesc>
        </biblFull>
      </listBibl>
    </body>
    <back>
      <listOrg type="structures">
        <org type="laboratory" xml:id="struct-93783" status="VALID">
          <idno type="IdRef">156733447</idno>
          <idno type="RNSR">200512540N</idno>
          <idno type="ROR">https://ror.org/05n4nmn13</idno>
          <idno type="Wikidata">Q30262319</idno>
          <orgName>Génétique moléculaire, génomique, microbiologie</orgName>
          <orgName type="acronym">GMGM</orgName>
          <date type="start">2009-01-01</date>
          <desc>
            <address>
              <addrLine>28 Rue Goethe 67083 STRASBOURG CEDEX</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://gmgm.unistra.fr</ref>
          </desc>
          <listRelation>
            <relation active="#struct-199013" type="direct"/>
            <relation name="UMR7156" active="#struct-441569" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-303306" status="VALID">
          <idno type="ROR">https://ror.org/01dr6c206</idno>
          <orgName>Polska Akademia Nauk = Polish Academy of Sciences = Académie polonaise des sciences</orgName>
          <orgName type="acronym">PAN</orgName>
          <date type="start">1951-10-01</date>
          <desc>
            <address>
              <addrLine>Pałac Kultury i Nauki, pl. Defilad 1, 00-901 Warszawa, skrytka pocztowa 24, Polska</addrLine>
              <country key="PL"/>
            </address>
            <ref type="url">https://www.pan.pl</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-199013" status="VALID">
          <idno type="IdRef">131056549</idno>
          <idno type="ROR">https://ror.org/00pg6eq24</idno>
          <orgName>Université de Strasbourg</orgName>
          <orgName type="acronym">UNISTRA</orgName>
          <desc>
            <address>
              <addrLine>4 rue Blaise Pascal - CS 90032 - 67081 Strasbourg cedex</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.unistra.fr/</ref>
          </desc>
        </org>
        <org type="regroupinstitution" xml:id="struct-441569" status="VALID">
          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <idno type="ROR">https://ror.org/02feahw73</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>
            <address>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.cnrs.fr/</ref>
          </desc>
        </org>
      </listOrg>
    </back>
  </text>
</TEI>