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Chapitre D'ouvrage Année : 2022

Preparation and Characterization of Heparan Sulfate-Derived Oligosaccharides to Investigate Protein–GAG Interaction and HS Biosynthesis Enzyme Activity

Résumé

Heparan sulfate chains are complex and structurally diverse polysaccharides that interact with a large number of proteins, thereby regulating a vast array of biological functions. Understanding this activity requires obtaining oligosaccharides of defined structures. Here we describe methods for isolating, engineering, and characterizing heparan sulfate-derived oligosaccharides and approaches based on high-performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR), and bio-layer interferometry (BLI) to study their structures, modifications, and interactions.
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Dates et versions

hal-03796550 , version 1 (04-10-2022)

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Cédric Laguri, Rabia Sadir, Evelyne Gout, Romain Vivès, Hugues Lortat-Jacob. Preparation and Characterization of Heparan Sulfate-Derived Oligosaccharides to Investigate Protein–GAG Interaction and HS Biosynthesis Enzyme Activity. Glycosaminoglycans, 2303, Springer US, pp.121-137, 2022, Methods in Molecular Biology, ⟨10.1007/978-1-0716-1398-6_11⟩. ⟨hal-03796550⟩
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