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Article Dans Une Revue Cell Année : 2022

Sustained deep-tissue voltage recording using a fast indicator evolved for two-photon microscopy

Zhuohe Liu
Xiaoyu Lu
  • Fonction : co premier-auteur
Vincent Villette
Yueyang Gou
Sharon Su
Saumil Patel
Cameron Smith
Andreas Tolias

Résumé

Genetically encoded voltage indicators are emerging tools for monitoring voltage dynamics with cell-type specificity. However, current indicators enable a narrow range of applications due to poor performance under two-photon microscopy, a method of choice for deep-tissue recording. To improve indicators, we developed a multiparameter high-throughput platform to optimize voltage indicators for two-photon microscopy. Using this system, we identified JEDI-2P, an indicator that is faster, brighter, and more sensitive and photostable than its predecessors. We demonstrate that JEDI-2P can report light-evoked responses in axonal termini of Drosophila interneurons and the dendrites and somata of amacrine cells of isolated mouse retina. JEDI-2P can also optically record the voltage dynamics of individual cortical neurons in awake behaving mice for more than 30 min using both resonant-scanning and ULoVE random-access microscopy. Finally, ULoVE recording of JEDI-2P can robustly detect spikes at depths exceeding 400 μm and report voltage correlations in pairs of neurons.
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Dates et versions

hal-03795400 , version 1 (21-11-2022)

Identifiants

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Zhuohe Liu, Xiaoyu Lu, Vincent Villette, Yueyang Gou, Kevin Colbert, et al.. Sustained deep-tissue voltage recording using a fast indicator evolved for two-photon microscopy. Cell, 2022, 185 (18), pp.3408-3425.e29. ⟨10.1016/j.cell.2022.07.013⟩. ⟨hal-03795400⟩
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