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Journal Articles Virus Research Year : 2014

Insights into RNA synthesis, capping, and proofreading mechanisms of SARS-coronavirus

Abstract

The successive emergence of highly pathogenic coronaviruses (CoVs) such as the Severe Acute Respiratory Syndrome (SARS-CoV) in 2003 and the Middle East Respiratory Syndrome Coronavirus (MERS-CoV) in 2012 has stimulated a number of studies on the molecular biology. This research has provided significant new insight into functions and activities of the replication/transcription multi-protein complex. The latter directs both continuous and discontinuous RNA synthesis to replicate and transcribe the large coronavirus genome made of a single-stranded, positive-sense RNA of similar to 30 kb. In this review, we summarize our current understanding of SARS-CoV enzymes involved in RNA biochemistry, such as the in vitro characterization of a highly active and processive RNA polymerase complex which can associate with methyltransferase and 3'-5' exoribonuclease activities involved in RNA capping, and RNA proofreading, respectively. The recent discoveries reveal fascinating RNA-synthesizing machinery, highlighting the unique position of coronaviruses in the RNA virus world.

Dates and versions

hal-03794372 , version 1 (03-10-2022)

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Marion Sevajol, Lorenzo Subissi, Etienne Decroly, Bruno Canard, Isabelle Imbert. Insights into RNA synthesis, capping, and proofreading mechanisms of SARS-coronavirus. Virus Research, 2014, 194, pp.90-99. ⟨10.1016/j.virusres.2014.10.008⟩. ⟨hal-03794372⟩
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