Objectives The present study explored the differences between athletes’ and parents’ perceptions of parental practices (i.e., active involvement, directive, pressure, praise, and understanding behaviours implemented by parents in the context of their child’s sport) by considering athletes’ and parents’ gender. Design cross-sectional study. Method Parents (N = 352) and athletes (N = 256, M = 14.72 years) completed a questionnaire to measure their perceptions of parental practices in sport. Zero-order correlations were computed for the entire sample and each sub-group (i.e., father-daughter, father-son, mother-daughter, and mother-son). One-level multilevel model (level 1: respondent) was computed to measure the influence of the respondent on the perceptions of parental practices. Two-level multilevel model (level 1: respondent, level 2: dyad) estimated the impact of the athletes’ gender, parents’ gender, and their interaction on the differences in perceptions. Results Correlations highlighted relatively modest concordance between perceptions of parents and adolescents. Multilevel models showed that compared to athletes, parents reported significantly less frequent use for directive behaviours (β = −0.29) and more frequent use for active involvement (β = −0.18) and praise and understanding (β = 0.27). Correlations and multilevel models showed that the differences between athletes’ and parents’ perceptions of parental practices differed according to gender. The differences in perceptions of the directive behaviours (β = −0.22) and active involvement (β = 0.22) were higher when the father was involved in the dyad compared to the mother.