Analytical Development for the Assessment of Pesticide Contaminations in Blood and Plasma of Wild Birds: The Case of Grey Partridges (Perdix Perdix) - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue SSRN Electronic Journal Année : 2023

Analytical Development for the Assessment of Pesticide Contaminations in Blood and Plasma of Wild Birds: The Case of Grey Partridges (Perdix Perdix)

Résumé

In this study, blood and plasma of grey partridges ( Perdix perdix) were analyzed to assess their potential contamination by plant protection products (PPP) and especially pesticide compounds.The group of pesticides selected is composed of a huge variety of compounds. Therefore, in this study, two methods were optimized and validated to analyze 104 compounds including herbicides, insecticides, fungicides and photoprotectors or synergists.Various extraction methods found in the literature were compared and adapted for the extraction of pesticides from blood and plasma. After extraction, samples were concentrated then injected for quantification simultaneously in LC-MS/MS and ATD-GC/MS/MS with an automatic thermal desorption step (ATD).Both LC-MS/MS and ATD-GC/MSMS analyses were performed using the MRM mode with 2 mass transitions for each compound.For both analytical methods, calibrations in triplicate were done by spiking within clean matrices using deuterated internal standard as ISTD. Evaluation of reproducibility, repeatability and determination of extraction yields were also performed with satisfactory results for most compounds then LOD and LOQ were determined with good sensibility results. LODs varied from 0.005 to 0.035 pg mg -1 and LOQs from 0.017 to 0.116 pg mg -1 for both matrices and methods.The two distinct analytical methods were then successfully applied to 70 blood samples and 35 plasma samples.
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hal-03792740 , version 1 (19-12-2022)

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Anaïs Rodrigues, Agathe Gaffard, Jérôme Moreau, Karine Monceau, Olivier Delhomme, et al.. Analytical Development for the Assessment of Pesticide Contaminations in Blood and Plasma of Wild Birds: The Case of Grey Partridges (Perdix Perdix). SSRN Electronic Journal, 2023, 1687, pp.463681. ⟨10.1016/j.chroma.2022.463681⟩. ⟨hal-03792740⟩
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