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Article Dans Une Revue Nature Communications Année : 2022

Resolving molecular diffusion and aggregation of antibody proteins with megahertz X-ray free-electron laser pulses

Mario Reiser
Anita Girelli
  • Fonction : Auteur
Anastasia Ragulskaya
  • Fonction : Auteur
Sudipta Das
  • Fonction : Auteur
Sharon Berkowicz
  • Fonction : Auteur
Maddalena Bin
Marjorie Ladd-Parada
Mariia Filianina
Hanna-Friederike Poggemann
  • Fonction : Auteur
Nafisa Begam
  • Fonction : Auteur
Mohammad Sayed Akhundzadeh
  • Fonction : Auteur
Sonja Timmermann
Lisa Randolph
Yuriy Chushkin
  • Fonction : Auteur
Tilo Seydel
Ulrike Boesenberg
  • Fonction : Auteur
Jörg Hallmann
  • Fonction : Auteur
Johannes Möller
  • Fonction : Auteur
Angel Rodriguez-Fernandez
Robert Rosca
Robert Schaffer
  • Fonction : Auteur
Markus Scholz
Roman Shayduk
  • Fonction : Auteur
Alexey Zozulya
Anders Madsen
Frank Schreiber
Fajun Zhang
  • Fonction : Auteur
Fivos Perakis
Christian Gutt

Résumé

Abstract X-ray free-electron lasers (XFELs) with megahertz repetition rate can provide novel insights into structural dynamics of biological macromolecule solutions. However, very high dose rates can lead to beam-induced dynamics and structural changes due to radiation damage. Here, we probe the dynamics of dense antibody protein (Ig-PEG) solutions using megahertz X-ray photon correlation spectroscopy (MHz-XPCS) at the European XFEL. By varying the total dose and dose rate, we identify a regime for measuring the motion of proteins in their first coordination shell, quantify XFEL-induced effects such as driven motion, and map out the extent of agglomeration dynamics. The results indicate that for average dose rates below 1.06 kGy μs −1 in a time window up to 10 μs, it is possible to capture the protein dynamics before the onset of beam induced aggregation. We refer to this approach as correlation before aggregation and demonstrate that MHz-XPCS bridges an important spatio-temporal gap in measurement techniques for biological samples.

Dates et versions

hal-03782881 , version 1 (21-09-2022)

Identifiants

Citer

Mario Reiser, Anita Girelli, Anastasia Ragulskaya, Sudipta Das, Sharon Berkowicz, et al.. Resolving molecular diffusion and aggregation of antibody proteins with megahertz X-ray free-electron laser pulses. Nature Communications, 2022, 13 (1), pp.5528. ⟨10.1038/s41467-022-33154-7⟩. ⟨hal-03782881⟩

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