Platelets Induce Thymic Stromal Lymphopoietin Production by Endothelial Cells: Contribution to Fibrosis in Human Systemic Sclerosis. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Arthritis and Rheumatology (hoboken, N.j.) Année : 2016

Platelets Induce Thymic Stromal Lymphopoietin Production by Endothelial Cells: Contribution to Fibrosis in Human Systemic Sclerosis.

Marie-Elise Truchetet
  • Fonction : Auteur
Béatrice Demoures
  • Fonction : Auteur
Jorge Eduardo Guimaraes
  • Fonction : Auteur
Anne Bertrand
Paôline Laurent
  • Fonction : Auteur
Isabelle Douchet
  • Fonction : Auteur
Clément Jacquemin
  • Fonction : Auteur
Liliane Khoryati
  • Fonction : Auteur
Pierre Duffau
  • Fonction : Auteur
Estibaliz Lazaro
  • Fonction : Auteur
Christophe Richez
Julien Seneschal
  • Fonction : Auteur
Marie-Sylvie Doutre
  • Fonction : Auteur
Jean-Luc Pellegrin
  • Fonction : Auteur
Joël Constans
  • Fonction : Auteur
Thierry Schaeverbeke
  • Fonction : Auteur
Patrick Blanco
  • Fonction : Auteur
Cécile Contin-Bordes
  • Fonction : Auteur

Résumé

OBJECTIVE: To investigate the relationship between vascular damage and fibrosis in systemic sclerosis (SSc) by testing the hypothesis that platelets contribute to skin fibrosis via the activation of human dermal microvascular endothelial cells (HDMECs) and subsequent production of profibrotic mediators. METHODS: A total of 203 SSc patients and 30 healthy donors were prospectively enrolled between 2012 and 2015 at the University Hospital of Bordeaux. Immunohistochemistry and immunofluorescence analyses were performed on skin biopsy sections from 18 SSc patients and 5 healthy donors. Serum thymic stromal lymphopoietin (TSLP) levels were measured by enzyme-linked immunosorbent assay in the entire cohort. HDMECs and fibroblasts were purified from biopsy sections. Extracellular matrix production by cultured fibroblasts was assessed by real-time quantitative polymerase chain reaction. RESULTS: Serum TSLP levels were significantly increased in SSc patients compared to healthy donors (P < 0.0001) and were associated with a higher frequency of vasculopathy (P = 0.02). The proportion of TSLP-positive dermal cells was increased in the skin of SSc patients compared with healthy donors (P < 0.0001) and was correlated with fibrosis (modified Rodnan skin thickness score) (r = 0.6146, P = 0.0001). In SSc dermis, TSLP was mainly expressed by CD31-positive endothelial cells. In vitro, activated platelets induced TSLP production by HDMECs in an interleukin-1β-dependent manner. SSc fibroblasts responded differently according to their original TSLP environment. CONCLUSION: Taken together, these results identify HDMECs as contributors to TSLP production in SSc and suggest a potential mechanism by which platelets may profoundly affect the fibrotic process in SSc.
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Dates et versions

hal-03778567 , version 1 (15-09-2022)

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Marie-Elise Truchetet, Béatrice Demoures, Jorge Eduardo Guimaraes, Anne Bertrand, Paôline Laurent, et al.. Platelets Induce Thymic Stromal Lymphopoietin Production by Endothelial Cells: Contribution to Fibrosis in Human Systemic Sclerosis.. Arthritis and Rheumatology (hoboken, N.j.), 2016, 68 (11), pp.2784-2794. ⟨10.1002/art.39817⟩. ⟨hal-03778567⟩

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