A case study to dissect immunity to SARS-CoV-2 in a neonate nonhuman primate model - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Frontiers in Immunology Année : 2022

A case study to dissect immunity to SARS-CoV-2 in a neonate nonhuman primate model

Natalia Nunez
  • Fonction : Auteur

Résumé

Most children are less severely affected by coronavirus-induced disease 2019 (COVID-19) than adults, and thus more difficult to study progressively. Here, we provide a neonatal nonhuman primate (NHP) deep analysis of early immune responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection in blood and mucosal tissues. In addition, we provide a comparison with SARS-CoV-2-infected adult NHP. Infection of the neonate resulted in a mild disease compared with adult NHPs that develop, in most cases, moderate lung lesions. In concomitance with the viral RNA load increase, we observed the development of an early innate response in the blood, as demonstrated by RNA sequencing, flow cytometry, and cytokine longitudinal data analyses. This response included the presence of an antiviral type-I IFN gene signature, a persistent and lasting NKT cell population, a balanced peripheral and mucosal IFN-γ/IL-10 cytokine response, and an increase in B cells that was accompanied with anti-SARS-CoV-2 antibody response. Viral kinetics and immune responses coincided with changes in the microbiota profile composition in the pharyngeal and rectal mucosae. In the mother, viral RNA loads were close to the quantification limit, despite the very close contact with SARS-CoV-2-exposed neonate. This pilot study demonstrates that neonatal NHPs are a relevant model for pediatric SARS-CoV-2 infection, permitting insights into the early steps of anti-SARS-CoV-2 immune responses in infants.
Fichier principal
Vignette du fichier
fov&1.pdf (6.41 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-03778360 , version 1 (16-09-2022)

Licence

Paternité

Identifiants

Citer

Claire-Maëlle Fovet, Camille Pimienta, Mathilde Galhaut, Francis Relouzat, Natalia Nunez, et al.. A case study to dissect immunity to SARS-CoV-2 in a neonate nonhuman primate model. Frontiers in Immunology, 2022, 13, pp.855230. ⟨10.3389/fimmu.2022.855230⟩. ⟨hal-03778360⟩
118 Consultations
32 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More