One of the main challenges in terms of public health concerns the prevention of bacterial contamination using rapid, highly sensitive and specific detection techniques. The development of highly sensitive bacterial sensors for Escherichia coli detection based on networks of silicon nanowires has been carried out in this work. The interest of these nano-objects takes advantage in a large contact surface allowing potentially important interactions with bacteria. Their presence induces a change in electrical interaction through the silicon nanowires array and is the basis for the development of silicon nanowires based electrical resistances acting as bacteria sensors. High specificity of these sensors is ensured by chemical functionalization of the nanowires allowing the binding of specific antibodies targeting the lipopolysaccharide (anti-LPS) of E. coli, but not S. aureus. The sensor displays a sensitivity of 83 μA per decade of CFU/mL due to the nanometric dimensions of the nanowires. The electrical measurements ensure the detection of various E. coli concentrations down to 102 CFU/mL. This SiNW biosensor device demonstrated its potential as an alternative tool for real-time bacterial detection as miniaturizable and low-cost integrated electronic sensor compatible with the classical silicon technology.