<?xml version="1.0" encoding="utf-8"?>
<TEI xmlns="http://www.tei-c.org/ns/1.0" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:hal="http://hal.archives-ouvertes.fr/" xmlns:gml="http://www.opengis.net/gml/3.3/" xmlns:gmlce="http://www.opengis.net/gml/3.3/ce" version="1.1" xsi:schemaLocation="http://www.tei-c.org/ns/1.0 http://api.archives-ouvertes.fr/documents/aofr-sword.xsd">
  <teiHeader>
    <fileDesc>
      <titleStmt>
        <title>HAL TEI export of hal-03771118</title>
      </titleStmt>
      <publicationStmt>
        <distributor>CCSD</distributor>
        <availability status="restricted">
          <licence target="https://creativecommons.org/publicdomain/zero/1.0/">CC0 1.0 - Universal</licence>
        </availability>
        <date when="2026-05-20T20:05:34+02:00"/>
      </publicationStmt>
      <sourceDesc>
        <p part="N">HAL API Platform</p>
      </sourceDesc>
    </fileDesc>
  </teiHeader>
  <text>
    <body>
      <listBibl>
        <biblFull>
          <titleStmt>
            <title xml:lang="en">Targeted insertion of reporter transgene into a gene safe harbor in human blood fluke, Schistosoma mansoni</title>
            <author role="aut">
              <persName>
                <forename type="first">Wannaporn Ittiprasert</forename>
                <surname>Tanno</surname>
              </persName>
              <email type="md5">570e38c0cf6ffd1aeb6a9818556bb5c6</email>
              <email type="domain">gwu.edu</email>
              <idno type="idhal" notation="numeric">1161904</idno>
              <idno type="halauthorid" notation="string">2579392-1161904</idno>
              <idno type="ORCID">https://orcid.org/0000-0001-9411-8883</idno>
              <affiliation ref="#struct-304728"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Max</forename>
                <forename type="middle">F</forename>
                <surname>Möscheid</surname>
              </persName>
              <idno type="halauthorid">2579393-0</idno>
              <affiliation ref="#struct-301926"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Cristian</forename>
                <surname>Chaparro</surname>
              </persName>
              <email type="md5">57c6d7c7a71199140a2e4544dab55967</email>
              <email type="domain">univ-perp.fr</email>
              <idno type="idhal" notation="string">cristian-chaparro</idno>
              <idno type="idhal" notation="numeric">172039</idno>
              <idno type="halauthorid" notation="string">4864-172039</idno>
              <idno type="RESEARCHERID">http://www.researcherid.com/rid/D-1427-2015</idno>
              <idno type="ORCID">https://orcid.org/0000-0002-5162-349X</idno>
              <idno type="IDREF">https://www.idref.fr/253122988</idno>
              <idno type="RESEARCHERID">http://www.researcherid.com/rid/http://www.researcherid.com/rid/D-1427-2015</idno>
              <affiliation ref="#struct-1100741"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Victoria</forename>
                <forename type="middle">H</forename>
                <surname>Mann</surname>
              </persName>
              <idno type="halauthorid">2579394-0</idno>
              <affiliation ref="#struct-304728"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Thomas</forename>
                <surname>Quack</surname>
              </persName>
              <idno type="halauthorid">1326111-0</idno>
              <affiliation ref="#struct-301926"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Rutchanee</forename>
                <surname>Rodpai</surname>
              </persName>
              <idno type="halauthorid">2579395-0</idno>
              <affiliation ref="#struct-304728"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Andre</forename>
                <surname>Miller</surname>
              </persName>
              <idno type="halauthorid">2579396-0</idno>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Prapakorn</forename>
                <surname>Wistiphongpun</surname>
              </persName>
              <idno type="halauthorid">2579397-0</idno>
              <affiliation ref="#struct-304728"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Watunyoo</forename>
                <surname>Buakaew</surname>
              </persName>
              <idno type="halauthorid">2579398-0</idno>
              <affiliation ref="#struct-304728"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Margaret</forename>
                <surname>Mentink-Kane</surname>
              </persName>
              <idno type="halauthorid">2579399-0</idno>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Legendré</forename>
                <surname>Miller</surname>
              </persName>
              <idno type="halauthorid">2579400-0</idno>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Sarah</forename>
                <surname>Schmid</surname>
              </persName>
              <idno type="halauthorid">2087533-0</idno>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Anastas</forename>
                <surname>Popratiloff</surname>
              </persName>
              <idno type="halauthorid">789516-0</idno>
              <affiliation ref="#struct-240805"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Christoph</forename>
                <forename type="middle">G</forename>
                <surname>Grevelding</surname>
              </persName>
              <email type="md5">2ad7d301cc58b4fce1133031e12afc48</email>
              <email type="domain">vetmed.uni-giessen.de</email>
              <idno type="idhal" notation="numeric">1108919</idno>
              <idno type="halauthorid" notation="string">2276061-1108919</idno>
              <idno type="ORCID">https://orcid.org/0000-0002-3144-2026</idno>
              <affiliation ref="#struct-301926"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Christoph</forename>
                <surname>Grunau</surname>
              </persName>
              <email type="md5">fa3ff922a470ba225fa71c81d69f5360</email>
              <email type="domain">univ-perp.fr</email>
              <idno type="idhal" notation="string">christoph-grunau</idno>
              <idno type="idhal" notation="numeric">171567</idno>
              <idno type="halauthorid" notation="string">5082-171567</idno>
              <idno type="RESEARCHERID">http://www.researcherid.com/rid/A-6989-2010</idno>
              <idno type="ORCID">https://orcid.org/0000-0002-2161-412X</idno>
              <idno type="IDREF">https://www.idref.fr/17660832X</idno>
              <idno type="RESEARCHERID">http://www.researcherid.com/rid/http://www.researcherid.com/rid/A-6989-2010</idno>
              <affiliation ref="#struct-1100741"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Paul</forename>
                <forename type="middle">J</forename>
                <surname>Brindley</surname>
              </persName>
              <email type="md5">877aec26d65918247cc89d07d4c1100e</email>
              <email type="domain">gwu.edu</email>
              <idno type="idhal" notation="numeric">1161905</idno>
              <idno type="halauthorid" notation="string">2579401-1161905</idno>
              <affiliation ref="#struct-304728"/>
            </author>
            <editor role="depositor">
              <persName>
                <forename>Anne</forename>
                <surname>Modat</surname>
              </persName>
              <email type="md5">aa4801e7909c9ac5fccd1a6a3ca461ff</email>
              <email type="domain">umontpellier.fr</email>
            </editor>
          </titleStmt>
          <editionStmt>
            <edition n="v1" type="current">
              <date type="whenSubmitted">2022-09-07 10:00:16</date>
              <date type="whenWritten">2022-09-02</date>
              <date type="whenModified">2024-11-07 16:14:04</date>
              <date type="whenReleased">2022-09-12 09:16:41</date>
              <date type="whenProduced">2022-09-02</date>
              <date type="whenEndEmbargoed">2022-09-07</date>
              <ref type="file" target="https://hal.science/hal-03771118v1/document">
                <date notBefore="2022-09-07"/>
              </ref>
              <ref type="file" subtype="author" n="1" target="https://hal.science/hal-03771118v1/file/Ittiprasert-2022-Biorxiv-Targeted-MANUSCRIT.pdf" id="file-3771118-3301091">
                <date notBefore="2022-09-07"/>
              </ref>
            </edition>
            <respStmt>
              <resp>contributor</resp>
              <name key="104000">
                <persName>
                  <forename>Anne</forename>
                  <surname>Modat</surname>
                </persName>
                <email type="md5">aa4801e7909c9ac5fccd1a6a3ca461ff</email>
                <email type="domain">umontpellier.fr</email>
              </name>
            </respStmt>
          </editionStmt>
          <publicationStmt>
            <distributor>CCSD</distributor>
            <idno type="halId">hal-03771118</idno>
            <idno type="halUri">https://hal.science/hal-03771118</idno>
            <idno type="halBibtex">tanno:hal-03771118</idno>
            <idno type="halRefHtml">2022</idno>
            <idno type="halRef">2022</idno>
            <availability status="restricted">
              <licence target="https://about.hal.science/hal-authorisation-v1/">HAL Authorization<ref corresp="#file-3771118-3301091"/></licence>
            </availability>
          </publicationStmt>
          <seriesStmt>
            <idno type="stamp" n="CNRS">CNRS - Centre national de la recherche scientifique</idno>
            <idno type="stamp" n="UNIV-PERP">Université Perpignan Via Domitia</idno>
            <idno type="stamp" n="IFREMER">IFREMER : Institut français de recherche pour l'exploitation de la mer</idno>
            <idno type="stamp" n="UNIV-MONTPELLIER">Université de Montpellier</idno>
            <idno type="stamp" n="IHPE" corresp="UNIV-MONTPELLIER">Interactions hôtes-pathologies-environnement</idno>
            <idno type="stamp" n="IHPE-2MAP">Mécanismes Moléculaires d'Adaptation et de Plasticité</idno>
            <idno type="stamp" n="UM-2015-2021" corresp="UNIV-MONTPELLIER">Université de Montpellier (2015-2021)</idno>
            <idno type="stamp" n="UM-EPE" corresp="UNIV-MONTPELLIER">Université de Montpellier - EPE</idno>
            <idno type="stamp" n="TEST3-HALCNRS">TEST3-HALCNRS</idno>
            <idno type="stamp" n="TEST4-HALCNRS">collection test</idno>
            <idno type="stamp" n="INEE-CNRS">Institut écologie et environnement du CNRS</idno>
          </seriesStmt>
          <notesStmt/>
          <sourceDesc>
            <biblStruct>
              <analytic>
                <title xml:lang="en">Targeted insertion of reporter transgene into a gene safe harbor in human blood fluke, Schistosoma mansoni</title>
                <author role="aut">
                  <persName>
                    <forename type="first">Wannaporn Ittiprasert</forename>
                    <surname>Tanno</surname>
                  </persName>
                  <email type="md5">570e38c0cf6ffd1aeb6a9818556bb5c6</email>
                  <email type="domain">gwu.edu</email>
                  <idno type="idhal" notation="numeric">1161904</idno>
                  <idno type="halauthorid" notation="string">2579392-1161904</idno>
                  <idno type="ORCID">https://orcid.org/0000-0001-9411-8883</idno>
                  <affiliation ref="#struct-304728"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Max</forename>
                    <forename type="middle">F</forename>
                    <surname>Möscheid</surname>
                  </persName>
                  <idno type="halauthorid">2579393-0</idno>
                  <affiliation ref="#struct-301926"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Cristian</forename>
                    <surname>Chaparro</surname>
                  </persName>
                  <email type="md5">57c6d7c7a71199140a2e4544dab55967</email>
                  <email type="domain">univ-perp.fr</email>
                  <idno type="idhal" notation="string">cristian-chaparro</idno>
                  <idno type="idhal" notation="numeric">172039</idno>
                  <idno type="halauthorid" notation="string">4864-172039</idno>
                  <idno type="RESEARCHERID">http://www.researcherid.com/rid/D-1427-2015</idno>
                  <idno type="ORCID">https://orcid.org/0000-0002-5162-349X</idno>
                  <idno type="IDREF">https://www.idref.fr/253122988</idno>
                  <idno type="RESEARCHERID">http://www.researcherid.com/rid/http://www.researcherid.com/rid/D-1427-2015</idno>
                  <affiliation ref="#struct-1100741"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Victoria</forename>
                    <forename type="middle">H</forename>
                    <surname>Mann</surname>
                  </persName>
                  <idno type="halauthorid">2579394-0</idno>
                  <affiliation ref="#struct-304728"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Thomas</forename>
                    <surname>Quack</surname>
                  </persName>
                  <idno type="halauthorid">1326111-0</idno>
                  <affiliation ref="#struct-301926"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Rutchanee</forename>
                    <surname>Rodpai</surname>
                  </persName>
                  <idno type="halauthorid">2579395-0</idno>
                  <affiliation ref="#struct-304728"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Andre</forename>
                    <surname>Miller</surname>
                  </persName>
                  <idno type="halauthorid">2579396-0</idno>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Prapakorn</forename>
                    <surname>Wistiphongpun</surname>
                  </persName>
                  <idno type="halauthorid">2579397-0</idno>
                  <affiliation ref="#struct-304728"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Watunyoo</forename>
                    <surname>Buakaew</surname>
                  </persName>
                  <idno type="halauthorid">2579398-0</idno>
                  <affiliation ref="#struct-304728"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Margaret</forename>
                    <surname>Mentink-Kane</surname>
                  </persName>
                  <idno type="halauthorid">2579399-0</idno>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Legendré</forename>
                    <surname>Miller</surname>
                  </persName>
                  <idno type="halauthorid">2579400-0</idno>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Sarah</forename>
                    <surname>Schmid</surname>
                  </persName>
                  <idno type="halauthorid">2087533-0</idno>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Anastas</forename>
                    <surname>Popratiloff</surname>
                  </persName>
                  <idno type="halauthorid">789516-0</idno>
                  <affiliation ref="#struct-240805"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Christoph</forename>
                    <forename type="middle">G</forename>
                    <surname>Grevelding</surname>
                  </persName>
                  <email type="md5">2ad7d301cc58b4fce1133031e12afc48</email>
                  <email type="domain">vetmed.uni-giessen.de</email>
                  <idno type="idhal" notation="numeric">1108919</idno>
                  <idno type="halauthorid" notation="string">2276061-1108919</idno>
                  <idno type="ORCID">https://orcid.org/0000-0002-3144-2026</idno>
                  <affiliation ref="#struct-301926"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Christoph</forename>
                    <surname>Grunau</surname>
                  </persName>
                  <email type="md5">fa3ff922a470ba225fa71c81d69f5360</email>
                  <email type="domain">univ-perp.fr</email>
                  <idno type="idhal" notation="string">christoph-grunau</idno>
                  <idno type="idhal" notation="numeric">171567</idno>
                  <idno type="halauthorid" notation="string">5082-171567</idno>
                  <idno type="RESEARCHERID">http://www.researcherid.com/rid/A-6989-2010</idno>
                  <idno type="ORCID">https://orcid.org/0000-0002-2161-412X</idno>
                  <idno type="IDREF">https://www.idref.fr/17660832X</idno>
                  <idno type="RESEARCHERID">http://www.researcherid.com/rid/http://www.researcherid.com/rid/A-6989-2010</idno>
                  <affiliation ref="#struct-1100741"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Paul</forename>
                    <forename type="middle">J</forename>
                    <surname>Brindley</surname>
                  </persName>
                  <email type="md5">877aec26d65918247cc89d07d4c1100e</email>
                  <email type="domain">gwu.edu</email>
                  <idno type="idhal" notation="numeric">1161905</idno>
                  <idno type="halauthorid" notation="string">2579401-1161905</idno>
                  <affiliation ref="#struct-304728"/>
                </author>
              </analytic>
              <monogr>
                <imprint/>
              </monogr>
              <idno type="biorxiv">2022.09.02.506379</idno>
              <idno type="doi">10.1101/2022.09.02.506379</idno>
              <ref target="https://www.biorxiv.org/content/10.1101/2022.09.02.506379v2" type="seeAlso"/>
            </biblStruct>
          </sourceDesc>
          <profileDesc>
            <langUsage>
              <language ident="en">English</language>
            </langUsage>
            <textClass>
              <classCode scheme="halDomain" n="sdv.mp.par">Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology</classCode>
              <classCode scheme="halDomain" n="sdv.bio">Life Sciences [q-bio]/Biotechnology</classCode>
              <classCode scheme="halTypology" n="UNDEFINED">Preprints, Working Papers, ...</classCode>
              <classCode scheme="halOldTypology" n="UNDEFINED">Preprints, Working Papers, ...</classCode>
              <classCode scheme="halTreeTypology" n="UNDEFINED">Preprints, Working Papers, ...</classCode>
            </textClass>
            <abstract xml:lang="en">
              <p>We identified genomic safe harbor sites (GSH) in the human blood fluke, Schistosoma mansoni and developed a CRISPR-focused protocol for insertion of a reporter transgene into a representative GSH. The protocol employed ribonuclear protein complexes of Cas9 nuclease, three overlapping guide RNAs, and phosphorothioate-modified, double stranded donor DNAs encoding green fluorescent protein driven by a strong endogenous promoter. Gene-editing efficiencies of &gt;20% and reporter transgene fluorescence of &gt;50% of gene-edited eggs were obtained by five days after CRISPR transfection. These methods and results advance functional genomics for multicellular parasites, and represent a tractable path towards transgenic schistosomes using homology directed repair catalyzed transgene insertion. Identification and characterization of GSH is expected to facilitate consistent transgene activity with neutral influence on the host cell genome and, concurrently, provide a privileged locus for transgene activity. This approach should be adaptable to platyhelminths generally.</p>
            </abstract>
          </profileDesc>
        </biblFull>
      </listBibl>
    </body>
    <back>
      <listOrg type="structures">
        <org type="institution" xml:id="struct-304728" status="VALID">
          <idno type="ROR">https://ror.org/00y4zzh67</idno>
          <orgName>The George Washington University</orgName>
          <orgName type="acronym">GW</orgName>
          <desc>
            <address>
              <addrLine>2121 Eye Street, NW , Washington, DC 20052</addrLine>
              <country key="US"/>
            </address>
            <ref type="url">https://www.gwu.edu</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-301926" status="VALID">
          <orgName>Justus-Liebig-Universität Gießen = Justus Liebig University</orgName>
          <orgName type="acronym">JLU</orgName>
          <date type="start">2017-01-24</date>
          <desc>
            <address>
              <addrLine>Ludwigstraße 23, 35390 Gießen</addrLine>
              <country key="DE"/>
            </address>
            <ref type="url">http://www.uni-giessen.de/index.html</ref>
          </desc>
        </org>
        <org type="laboratory" xml:id="struct-1100741" status="VALID">
          <idno type="IdRef">189954582</idno>
          <idno type="ISNI">0000000403840099</idno>
          <idno type="RNSR">200711909U</idno>
          <idno type="ROR">https://ror.org/00zn13224</idno>
          <orgName>Interactions Hôtes-Pathogènes-Environnements</orgName>
          <orgName type="acronym">IHPE</orgName>
          <date type="start">2015-01-01</date>
          <desc>
            <address>
              <addrLine>Université de Perpignan Via Domitia Bâtiment R 52 Avenue Paul Alduy 66860 Perpignan cedex (Languedoc-Roussillon)</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://ihpe.univ-perp.fr/</ref>
          </desc>
          <listRelation>
            <relation active="#struct-101475" type="direct"/>
            <relation active="#struct-300022" type="direct"/>
            <relation name="UMR5244" active="#struct-441569" type="direct"/>
            <relation active="#struct-1100589" type="direct"/>
          </listRelation>
        </org>
        <org type="laboratory" xml:id="struct-240805" status="INCOMING">
          <orgName>Department of Anatomy and Regenerative Biology</orgName>
          <desc>
            <address>
              <addrLine>2121 I St NW, Washington, DC 20052</addrLine>
              <country key="US"/>
            </address>
          </desc>
          <listRelation>
            <relation active="#struct-304728" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-101475" status="VALID">
          <idno type="ROR">https://ror.org/03am2jy38</idno>
          <orgName>Université de Perpignan Via Domitia</orgName>
          <orgName type="acronym">UPVD</orgName>
          <desc>
            <address>
              <addrLine>52 avenue Paul Alduy - 66860 Perpignan Cedex 9</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.univ-perp.fr/</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-300022" status="VALID">
          <idno type="IdRef">027783073</idno>
          <idno type="ISNI">0000000121634107</idno>
          <idno type="ROR">https://ror.org/044jxhp58</idno>
          <orgName>Institut Français de Recherche pour l'Exploitation de la Mer</orgName>
          <orgName type="acronym">IFREMER</orgName>
          <date type="start">1984-01-01</date>
          <desc>
            <address>
              <country key="FR"/>
            </address>
            <ref type="url">https://wwz.ifremer.fr/</ref>
          </desc>
        </org>
        <org type="regroupinstitution" xml:id="struct-441569" status="VALID">
          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <idno type="ROR">https://ror.org/02feahw73</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>
            <address>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.cnrs.fr/</ref>
          </desc>
        </org>
        <org type="regroupinstitution" xml:id="struct-1100589" status="VALID">
          <idno type="ROR">https://ror.org/051escj72</idno>
          <orgName>Université de Montpellier</orgName>
          <orgName type="acronym">UM</orgName>
          <date type="start">2022-01-01</date>
          <desc>
            <address>
              <addrLine>163 rue Auguste Broussonnet - 34090 Montpellier</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.umontpellier.fr/</ref>
          </desc>
        </org>
      </listOrg>
    </back>
  </text>
</TEI>