Redox-labelled electrochemical aptasensors with nanosupported cancer cells - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Biosensors and Bioelectronics Année : 2022

Redox-labelled electrochemical aptasensors with nanosupported cancer cells

Résumé

The transfer of redox-labelled bioelectrochemical sensors from proteins to cells is not straightforward because of the cell downward force issue on the surface of the sensors. In this paper, 20-nm-thick nanopillars are introduced to overcome this issue, in a well-controlled manner. We show on both molecular dynamics simulations and experiments that suspending cells a few nanometers above an electrode surface enables redox-labelled tethered DNA aptamer probes to move freely, while remaining at an interaction distance from a target membrane protein, i. e. epithelial cell adhesion molecule (EpCAM), which is typically overexpressed in cancer cells. By this nanopillar configuration, the interaction of aptamer with cancer cells is clearly observable, with 13 cells as the lower limit of detection. Nanoconfinement induced by the gap between the electrode surface and the cell membrane appears to improve the limit of detection and to lower the melting temperature of DNA aptamer hairpins, offering an additional degree of freedom to optimize molecular recognition mechanisms. This novel nanosupported electrochemical DNA cell sensor scheme including Brownian-fluctuating redox species opens new opportunities for the design of all-electrical sensors using redox-labelled probes.
Fichier principal
Vignette du fichier
Manuscript-S. Li et al-R2-yc.pdf (1.07 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-03768941 , version 1 (10-01-2023)

Identifiants

Citer

S. Li, Yannick Coffinier, C. Lagadec, Fabrizio Cleri, K. Nishiguchi, et al.. Redox-labelled electrochemical aptasensors with nanosupported cancer cells. Biosensors and Bioelectronics, 2022, 216, 114643, 7 p. ⟨10.1016/j.bios.2022.114643⟩. ⟨hal-03768941⟩
54 Consultations
75 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More