Callose Detection and Quantification at Plasmodesmata in Bryophytes - Archive ouverte HAL Accéder directement au contenu
Chapitre D'ouvrage Année : 2022

Callose Detection and Quantification at Plasmodesmata in Bryophytes

Résumé

In bryophytes (i.e. mosses, liverworts and hornworts), extant representatives of early land plants, plasmodesmata have been described in a wide range of tissues. Although their contribution to bryophyte morphogenesis remains largely unexplored, several recent studies have suggested that the deposition of callose around plasmodesmata might regulate developmental and physiological responses in mosses. Here, we provide a protocol to image and quantify callose levels in the filamentous body of the model moss Physcomitrium (Physcomitrella) patens and discuss possible alternatives and pitfalls. More generally, this protocol establishes a framework to explore the distribution of callose in other bryophytes.
Fichier principal
Vignette du fichier
Muller_MiMB-final.pdf (117.07 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-03764627 , version 1 (01-09-2022)

Identifiants

Citer

Arthur Muller, Tomomichi Fujita, Yoan Coudert. Callose Detection and Quantification at Plasmodesmata in Bryophytes. Plasmodesmata, 2457, Springer US, pp.177-187, 2022, Methods in Molecular Biology, ⟨10.1007/978-1-0716-2132-5_11⟩. ⟨hal-03764627⟩
33 Consultations
286 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More