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Article Dans Une Revue IFAC Proceedings Volumes Année : 2011

Towards Real-Time Control of Gene Expression: in silico Analysis*

Jannis Uhlendorf
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Pascal Hersen
Gregory Batt
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Résumé

Abstract Genetic programs operating in a history-dependent fashion are ubiquitous in nature and govern sophisticated processes such as development and differentiation. The ability to systematically and predictably encode such programs would advance the engineering of synthetic organisms and ecosystems with rich signal processing abilities. Here we implement robust, scalable history-dependent programs by distributing the computational labor across a cellular population. Our design is based on standardized recombinase-driven DNA scaffolds expressing different genes according to the order of occurrence of inputs. These multicellular computing systems are highly modular, do not require cell-cell communication channels, and any program can be built by differential composition of strains containing well-characterized logic scaffolds. We developed automated workflows that researchers can use to streamline program design and optimization. We anticipate that the history-dependent programs presented here will support many applications using cellular populations for material engineering, biomanufacturing and healthcare.

Dates et versions

hal-03761737 , version 1 (26-08-2022)

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Jannis Uhlendorf, Pascal Hersen, Gregory Batt. Towards Real-Time Control of Gene Expression: in silico Analysis*. IFAC Proceedings Volumes, 2011, 44 (1), pp.14844-14850. ⟨10.3182/20110828-6-IT-1002.02614⟩. ⟨hal-03761737⟩
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