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Poster De Conférence Année : 2022

Whole brain mapping of activity during paradoxical sleep and wakefulness using TRAP mice and improved double fluorescence method and automatic counting

Résumé

Paradoxal sleep (PS) exhibit EEG activity in cortical structures similar to that observed during wakefulness. However, functional mapping of the human brain reveals that PS after dreams recall have a particular state- related neurodynamics. Corroborating these findings, our last results showed that only a few limbic cortical structures are activated during PS in contrast to wakefulness. Altogether, it confirms our hypotheses that neuronal activity during PS plays a specific role. Here, associating TRAP mice model and automatic counting of cFos and tdTomato + neurons we propose a novel approach to broaden the avenue in the study of the function of PS. Aim :1) confirm using the new methodology that PS and wakefulness are two different states; 2) determine the pattern of neuronal activation seen during PS compared to W and 3) verify if PS-PS mice depict more double labelled neurons than PS-W and W-PS mice. Seven TRAP2 mice were analysed in PS-W (n=1), W-PS (n=2) and PS-PS (n=4) conditions 1 week apart. Basal sleep wake cycle (SWC) recordings were performed 24h / day (10 - 10 am) in order to generate the automatic detection of PS as previously described herein (Arthaud, 2020). Mice underwent automatic PS deprivation during 48 h and received hydroxytomaxifen (4-OHT) 2 hours after the first epoch of PS, in the first condition; or perfusion, in the second condition. Brain sections (30 μm; cFos, Alexa 488; tdtomato, spontaneous fluorescence ~555 nm wavelength) were taken every 240 μm. The experiments were approved by the Ethics Research Committee of University Lyon 1 in accordance with the European guidelines for care in animal research (#21351). The development of a new method combining the use of TRAP mice with automatic counting open a path to the study over the whole brain of populations of neurons activated during two different conditions. Further, our results indicate that W and PS are completely different states during which different populations of neurons are activated.
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Dates et versions

hal-04176890 , version 1 (03-08-2023)

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  • HAL Id : hal-04176890 , version 1

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Renato Maciel, Sébastien Cabrera, Olivier Raineteau, Nathalie Mandairon, Patrice Fort, et al.. Whole brain mapping of activity during paradoxical sleep and wakefulness using TRAP mice and improved double fluorescence method and automatic counting. FENS Meeting, Jul 2022, PARIS, France. ⟨hal-04176890⟩
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