Click-Chemistry-Based Biomimetic Ligands Efficiently Capture G-Quadruplexes <i>In Vitro</i> and Help Localize Them at DNA Damage Sites in Human Cells - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue JACS Au Année : 2022

Click-Chemistry-Based Biomimetic Ligands Efficiently Capture G-Quadruplexes In Vitro and Help Localize Them at DNA Damage Sites in Human Cells

Résumé

Interrogating G-quadruplex (G4) biology at its deepest roots in human cells relies on the design, synthesis, and use of ever smarter molecular tools. Here, we demonstrate the versatility of biomimetic G4 ligands referred to as TASQ (template assembled synthetic G-quartet) in which a biotin handle was incorporated for G4-focused chemical biology investigations. We have rethought the biotinylated TASQ design to make it readily chemically accessible via an efficient click-chemistry-based strategy. The resulting biotinylated, triazole-assembled TASQ, or Bio-TriazoTASQ, was thus shown to efficiently isolate both DNA and RNA G4s from solution by affinity purification protocols, for identification purposes. Its versatility was then further demonstrated by optical imaging that provided unique mechanistic insights into the actual strategic relevance of G4-targeting strategies, showing that ligand-stabilized G4 sites colocalize with and, thus, are responsible for DNA damage foci in human cells.

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Génétique Chimie
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Dates et versions

hal-03738327 , version 1 (26-07-2022)

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Francesco Rota Sperti, Baptiste Dupouy, Jérémie Mitteaux, Angélique Pipier, Marc Pirrotta, et al.. Click-Chemistry-Based Biomimetic Ligands Efficiently Capture G-Quadruplexes In Vitro and Help Localize Them at DNA Damage Sites in Human Cells. JACS Au, 2022, 2, pp.1588 - 1595. ⟨10.1021/jacsau.2c00082⟩. ⟨hal-03738327⟩
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