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Communication Dans Un Congrès Année : 2022

The secretome of equine bone marrow-derived mesenchymal stem cells enhanced regenerative phenotype of equine articular chondrocytes

Résumé

Equine osteoarthritis (OA) leads to cartilage degradation with impaired animal well-being, premature cessation of sport activity, and financial losses. Many hopes lie on mesenchymal stem cell (MSC)-based therapies to repair cartilage, but these techniques face limitations inherent to cell itself. Soluble mediators and extracellular vesicles (EVs) secreted by MSCs are the alternative to overcome those limitations while preserving MSC restorative properties (Cosenza et al., 2017). The aim of this study was to assess the effect of equine bone marrow MSC secretome on equine articular chondrocytes (eACs) by indirect co-culture and/or MSC-conditioned media (CM) containing EVs. The expression of healthy cartilage/OA, and proliferation markers was evaluated in eACs cultured in monolayers or as 3D organoids. In vitro repair experiments with MSC-CM were made to evaluate the proliferation and migration of eACs. Our results demonstrated that MSC secretome influences eAC phenotype by increasing cartilage functionality markers such as Prg4, collagens and proliferation associated molecule in a greater way than MSCs. Cell migration was also enhanced, which could delay OA final outcomes. To confirm the presence of nanosized EVs in MSC-CM, nanoparticle tracking assay, transmission electron microscopy and exosome-specific markers detection were assessed. Lastly, fluorescence staining revealed effective uptake of exosomes by eAC. This study makes acellular therapy an appealing strategy to improve equine OA treatments. However, MSC secretome contains a wide variety of soluble mediators and small EVs, such as exosomes, and further investigation must be performed to understand the mechanisms occurring behind these promising effects.
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Dates et versions

hal-03728083 , version 1 (20-07-2022)

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  • HAL Id : hal-03728083 , version 1

Citer

Manon Jammes, Romain Contentin, Frédéric Cassé, Bastien Bourdon, Arnaud Bianchi, et al.. The secretome of equine bone marrow-derived mesenchymal stem cells enhanced regenerative phenotype of equine articular chondrocytes. Journées de l'Ecole Doctorale Normande de Biologie Intégrative, Santé, Environnement (nBISE) - 24e édition, Jun 2022, Caen, France. ⟨hal-03728083⟩
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