Identification of new Dickeya dadantii virulence factors secreted by the type 2 secretion system - Archive ouverte HAL Accéder directement au contenu
Poster De Conférence Année : 2022

Identification of new Dickeya dadantii virulence factors secreted by the type 2 secretion system

Lulu Liu
  • Fonction : Auteur
Christine Rascle
  • Fonction : Auteur
Agnès Rodrigue
B. Le Derout
  • Fonction : Auteur
Guy Condemine

Résumé

Dickeya are plant pathogenic bacteria able to provoke disease on a wide range of plants. A type 2 secretion system (T2SS) named Out is necessary for bacterial virulence. Its study in D. dadantii showed that it secretes a wide range of plant cell wall degrading enzymes, including pectinases and a cellulase. However, the full repertoire of exoproteins it can secrete has probably not yet been identified. Secreted proteins are first addressed to the periplasm before their secretion by Out. No secretion signal present on the protein allows the identification of substrates of a T2SS. To identify new Out substrates, we analyzed D. dadantii transcriptome data obtained in plant infection condition and searched for genes strongly induced encoding a protein with a signal sequence. We identified five new Out-secreted proteins: the iron-binding protein IbpS, the expansin YoaJ, the putative virulence factor VirK and two proteins of the DUF 4879 family, SvfA and SvfB. We showed that IbpS, SvfA and SvfB are required for full virulence of D. dadantii. Homologues of IbpS are present in other phytopathogenic microorganisms (fungi and oomycetes) where they also play a role in virulence. svf genes are present in a variable number of copies in other Pectobacteriaceae, up to three in D. fanghzongdai. This work opens the way to the study of the role of non-pectinolytic proteins secreted by the Out pathway in Pectobacteriaceae.

Domaines

Bactériologie
Fichier principal
Vignette du fichier
poster Assisie.pdf (3.4 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-03721396 , version 1 (19-07-2022)

Identifiants

  • HAL Id : hal-03721396 , version 1

Citer

Lulu Liu, Christine Rascle, Isabelle R. Gonçalves, Nathalie Poussereau, Agnès Rodrigue, et al.. Identification of new Dickeya dadantii virulence factors secreted by the type 2 secretion system. 14th international conference on plant pathogenic bacteria, Jul 2022, Assise, Italy. ⟨hal-03721396⟩
81 Consultations
13 Téléchargements

Partager

Gmail Facebook X LinkedIn More