Container material dictates stability of bacteriophage suspensions: Light scattering and infectivity measurements reveal mechanisms of infectious titre decay

Aims: To measure the infectious titer (IT) decay rate for various bacteriophages as a function of storage container material. Additionally, parallel light scattering and infectious titer measurements reveal distinct mechanisms for IT loss, depending on phage. Methods and Results: Suspensions of bacteriophages 44AHJD, P68, and gh-1 were stored in various labware. IT of each suspension was repeatedly measured over the course of two weeks. Large variability in IT decay was observed, with >4 log10 loss in glass and low-binding polypropylene.

Mots clés

Bacteriophage colloidal stability aggregation virus adsorption nanoparticle tracking analysis infectious titer phage stability plaque counting

Domaines

Biotechnologies Virologie

Fichier principal

J of Applied Microbiology - 2022 - O Connell - Container Material Dictates Stability of Bacteriophage Suspensions Light.pdf (1.03 Mo)

Origine	Fichiers produits par l'(les) auteur(s)

Yoann ROUPIOZ : Connectez-vous pour contacter le contributeur

https://hal.science/hal-03720069

Soumis le : lundi 11 juillet 2022-16:37:43

Dernière modification le : lundi 9 décembre 2024-03:30:40

Dates et versions

hal-03720069 , version 1 (11-07-2022)

Identifiants

HAL Id : hal-03720069 , version 1
DOI : 10.1111/jam.15581

Citer

Larry O'Connell, Yoann Roupioz, Pierre R Marcoux. Container material dictates stability of bacteriophage suspensions: Light scattering and infectivity measurements reveal mechanisms of infectious titre decay. Journal of Applied Microbiology, 2022, 133 (2), pp.529-543. ⟨10.1111/jam.15581⟩. ⟨hal-03720069⟩

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