Article Dans Une Revue Chemical Communications Année : 2022

Charge detection mass spectrometry on human-amplified fibrils from different synucleinopathies

Résumé

Amyloid fibrils are self-assembled mesoscopic protein aggregates, which can accumulate to form deposits or plaques in the brain. In vitro amplification of fibrils can be achieved with real-time quaking-induced conversion (RT-QuIC). However, this emerging technique would benefit from a complementary method to assess structural properties of the amplification products. This work demonstrates the feasibility of nanospray-charge-detection-mass-spectrometry (CDMS) performed on α-synuclein (αSyn) fibrils amplified from human brains with Parkinson's disease (PD) or Dementia with Lewy bodies (DLB) and its synergistic combination with RT-QuIC.

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hal-03716055 , version 1 (07-07-2022)

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Aikaterini Tsirkou, Flora Kaczorowski, Mathieu Verdurand, Rana Raffoul, Jonathan Pansieri, et al.. Charge detection mass spectrometry on human-amplified fibrils from different synucleinopathies. Chemical Communications, 2022, 58 (51), pp.7192-7195. ⟨10.1039/D2CC00200K⟩. ⟨hal-03716055⟩
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