Nuclear pore complex acetylation regulates mRNA export and cell cycle commitment in budding yeast - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue EMBO Journal Année : 2022

Nuclear pore complex acetylation regulates mRNA export and cell cycle commitment in budding yeast

Résumé

Abstract Nuclear pore complexes (NPCs) mediate communication between the nucleus and the cytoplasm and regulate gene expression by interacting with transcription and mRNA export factors. Lysine acetyl-transferases (KATs) promote transcription through acetylation of chromatin-associated proteins. We find that Esa1, the KAT subunit of the yeast NuA4 complex, also acetylates the nuclear pore basket component Nup60 to promote mRNA export. Acetylation of Nup60 recruits mRNA export factors to the nuclear basket, including the scaffolding subunit of the Transcription and Export 2 (TREX-2) complex, Sac3. Esa1-dependent nuclear export of mRNAs promotes entry into S phase, and is inhibited by the Hos3 deacetylase in G1 daughter cells to restrain their premature commitment to a new cell division cycle. This mechanism also inhibits expression of the nutrient-regulated GAL1 gene specifically in daughter cells. These results reveal how acetylation contributes to the functional plasticity of NPCs in specific cell types, and demonstrate how the evolutionarily conserved NuA4 complex regulates gene expression dually at the level of transcription and mRNA export, by modifying the nucleoplasmic entrance to nuclear pores.
Fichier principal
Vignette du fichier
The EMBO Journal - 2022 - Gomar‐Alba - Nuclear pore complex acetylation regulates mRNA export and cell cycle commitment in.pdf (9.95 Mo) Télécharger le fichier
Origine : Publication financée par une institution
Licence : CC BY - Paternité

Dates et versions

hal-03710644 , version 1 (09-03-2023)

Identifiants

Citer

Mercè Gomar-Alba, Vasilisa Pozharskaia, Bogdan Cichocki, Celia Schaal, Arun Kumar, et al.. Nuclear pore complex acetylation regulates mRNA export and cell cycle commitment in budding yeast. EMBO Journal, 2022, ⟨10.15252/embj.2021110271⟩. ⟨hal-03710644⟩
293 Consultations
64 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More