Comparison of the effects of two therapeutic strategies based on olfactory ensheathing cell transplantation and repetitive magnetic stimulation after spinal cord injury in female mice
Résumé
Spinal cord injury (SCI) is a debilitating condition, which leads to a permanent loss of functions below the injury site. The events which take place after SCI are characterized by cellular death, release of inhibitory factors, and inflammation. Many therapies have been studied to cure SCI, among them magnetic stimulation aims to reduce the secondary damages in particular by decreasing apoptosis, while, cellular transplantation promotes neuroregeneration by enhancing axonal regrowth. In the present study, we compared individually primary olfactory ensheathing cell (OEC) transplantation and repetitive trans-spinal magnetic stimulation (rTSMS) and then, we combined these two therapeutic approaches on tissue repair and functional recovery after SCI. To do so, SCIs were performed at Th10 level on female C57BL/6 mice, which were randomized into four groups: SCI, SCI + primary bOECs, SCI + STM, SCI + primary bulbar olfactory ensheathing cells (bOECs) + stimulation (STM). On these animals bioluminescence, immunohistological, and behavioral experiments were performed after SCI. Our results show that rTSMS has beneficial effect on the modulation of spinal scar by reducing fibrosis, demyelination, and microglial cell activation and by increasing the astroglial component of the scar, while, primary bOEC transplantation decreases microglial reactivity. At the opposite, locotronic experiments show that both treatments induce functional recovery. We did not observed any additional effect by combining the two therapeutic approaches. Taken together, the present study indicates that primary bOEC transplantation and rTSMS treatment act through different mechanisms after SCI to induce functional recovery. In our experimental paradigm, the combination of the two therapies does not induce any additional benefit.
Mots clés
RRID:AB_10563302: PDGFRβ
Abcam
ab91066
RRID:AB_10643424: PE
poly4064
BioLegend
406408
RRID:AB_2313568: Jackson ImmunoResearch
711-166-152
RRID:AB_2340667: Jackson ImmunoResearch
712-165-153
RRID:AB_2340812: Jackson ImmunoResearch
715-165-140
RRID:AB_2715913: Alexa 488
MRG2b-85
RRID:AB_306827: p75
ab8874
RRID:AB_476889: GFAP Cy3-conjugated Sigma-Aldrich
C9205
RRID:AB_777165:P DGFRβAbcam ab32570
RRID:AB_839504: Iba1
Wako
019-19741
RRID:AB_94975: MBP
Millipore
MAB386
RRID:IMSR_JAX:008450: L2G85Chco+/+ (FVB-Tg(CAG-luc
-GFP)L2G85Chco/J)
glial scar
magnetic stimulation
olfactory ensheathing cells and neuroregeneration
rehabilitation
spinal cord injury