Scattering correcting wavefront shaping for three-photon microscopy - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Optics Letters Année : 2022

Scattering correcting wavefront shaping for three-photon microscopy

Résumé

Three-photon (3P) microscopy is getting traction due to its superior performance in deep tissues. Yet, aberrations and light scattering still pose one of the main limitations in the attainable depth ranges for high-resolution imaging. Here, we show scattering correcting wavefront shaping with a simple continuous optimization algorithm, guided by the integrated 3P fluorescence signal. We demonstrate focusing and imaging behind scattering layers and investigate convergence trajectories for different sample geometries and feedback non-linearities. Furthermore, we show imaging through a mouse skull and demonstrate a novel fast phase estimation scheme that substantially increases the speed at which the optimal correction can be found.
Fichier principal
Vignette du fichier
main_revtex.pdf (2.97 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-03707080 , version 1 (28-06-2022)

Identifiants

Citer

Bernhard Rauer, Hilton B de Aguiar, Laurent Bourdieu, Sylvain Gigan. Scattering correcting wavefront shaping for three-photon microscopy. Optics Letters, 2022, 47 (23), pp.6233. ⟨10.1364/OL.468834⟩. ⟨hal-03707080⟩
48 Consultations
38 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More