Autofluorescence Loss in Photobleaching for Human Dentin ex vivo
Résumé
Two-photon fluorescence microscopy was performed on the enamel-dentin junction area of a human tooth using a femtosecond pulsed laser. We obtained a clear image contrast between the bright dentin and dark tubules with the autofluorescence generated from the endogenous fluorophores in dentin. The autofluorescence shows a broad spectrum due to complex cross links between dentinal collagens, which extend from blue to orange wavelengths (470–590 nm), but a gradual autofluorescence loss in pho- tobleaching was observed for a long-term exposure under strong excitation. For increasing excitation power, we found that two-step decay becomes significant in the spectrally integrated autofluorescence.
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Cite 10.3807/COPP.2022.6.1.086 Article Lee, S. G., Kim, M., Jeong, S., Hwang, J., Kim, J., Gourrier, A., Vial, J. C., & Kyhm, K. (2022). Autofluorescence Loss in Photobleaching for Human Dentin ex vivo. Current Optics and Photonics, 6(1), 86–91. https://doi.org/10.3807/COPP.2022.6.1.086