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Journal Articles Journal of biotechnology Year : 2011

Using Vaccinia's innate ability to introduce DNA into mammalian cells for production of recombinant proteins

Abstract

Production of recombinant protein in mammalian cells is time-consuming, labor-intensive and costly. While seeking to overcome these limitations, we discovered that Vaccinia virus has the innate ability to transfer exogenous plasmid DNA into mammalian cells during the infection process. Parameters influencing the efficiency of this event were characterized and a quick, simple and inexpensive way to produce eukaryotic proteins was established.

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Genetics
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hal-03702227 , version 1 (22-06-2022)

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B Jester, Robert Drillien, Marc Ruff, Catherine Florentz. Using Vaccinia's innate ability to introduce DNA into mammalian cells for production of recombinant proteins. Journal of biotechnology, 2011, 156 (3), pp.211-213. ⟨10.1016/j.jbiotec.2011.09.013⟩. ⟨hal-03702227⟩
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