Assessment of comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry based methods for investigating 206 dioxin-like micropollutants in animal-derived food matrices - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Chromatography A Année : 2015

Assessment of comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry based methods for investigating 206 dioxin-like micropollutants in animal-derived food matrices

Résumé

This paper evaluates different multiresidue methods based on comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry (GC × GC-TOF/MS) to analyze dioxin-related micropollutants in complex food matrices. In a first step, the column sets Rtx-PCB/BPX-50 and Rtx-Dioxin2/BPX-50 were compared in terms of peak shape (width and symmetry) and resolution for the separation of polychlorinated biphenyls (PCBs) and polychlorinated dibenzo-p-dioxins/dibenzofurans (PCDD/Fs) in solvent. A satisfactory separation of 206 dioxin-related micropollutants including the 17 toxic PCDD/Fs was achieved in 75 min with the column set Rtx-Dioxin2/BPX-50. In a second time, the GC × GC-TOF/MS method was spread to the analysis of dioxin-related micropollutants in complex food matrices. An extraction procedure including accelerated solvent extraction (ASE), centrifugal evaporation and gel permeation chromatography (GPC) was optimized. Starting with meat as a model matrix, a micropollutant spiking method was then set up by comparing seven methods in terms of recoveries and reproducibility. The method combining immersion of the meat in a large volume of solvent containing micropollutants followed by homogenization by blender induced recoveries in the acceptable range of 70-130% and satisfactory standard deviations (≤10%) for most of the compounds studied. Limits of detection of the GC × GC-TOF/MS method ranged between 50 and 100 pg/g of spiked fresh meat for PCBs and between 65 and 227 pg/g for PCDD/Fs. Potential applications of this method are discussed.
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hal-03696520 , version 1 (09-05-2023)

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Christelle Planche, Jérémy Ratel, Frédéric Mercier, Patrick Blinet, Laurent Debrauwer, et al.. Assessment of comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry based methods for investigating 206 dioxin-like micropollutants in animal-derived food matrices. Journal of Chromatography A, 2015, 1392, pp.74-81. ⟨10.1016/j.chroma.2015.02.054⟩. ⟨hal-03696520⟩
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