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Article Dans Une Revue Current Directions in Biomedical Engineering Année : 2022

Experimental setup for the ultrasonic fractionation of flowing whole blood in a capillary

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If a long ultrasound driving pulse is chosen such, that its frequency is greater than the resonance frequency of one cell type and less than the resonance frequency of another cell type, the cells are separated in different nodes and antinodes of the standing sound field. The purpose of this study was to build an experimental setup for the ultrasonic fractionation of whole blood in flow conditions. Blood fractionation is the separation of whole blood into its separate components, commonly used for analysis and transfusions. The experimental setup comprised a container with single-element ultrasound transducers customisable in elevation and azimuth, focussed on a capillary through which blood was flowing. The acoustic focus coincided with the optical focus of a microscopic system to which a high-speed camera was attached. Ultrasound transducers were used with centre frequencies between 1 MHz and 20 MHz and with pressure amplitudes less than 1 MPa. The duty cycle was greater than 10% in all experiments. The field of view was 0.2$\times$0.2 mm$^2$. The optical resolution and camera speed allowed for visibility of red and white blood cells during flow conditions. Preliminary results show that red and white blood cells could be forced to move in opposite directions. The acoustic frequencies and pressures used were representative for those used in clinical diagnosis.We have shown that ultrasonic whole blood fractionation is feasible using ultrasound. Sonic fractionation speed might be an indicator for diseases such as malaria.
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hal-03696320 , version 1 (02-09-2022)

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Charl Smalberger, Malavika Nathan, David Milton Rubin, Marietha Nel, Spiros Kotopoulis, et al.. Experimental setup for the ultrasonic fractionation of flowing whole blood in a capillary. Current Directions in Biomedical Engineering, 2022, Proceedings of the 2022 Joint Annual Conference of the Austrian (ÖGBMT), German (VDE DGBMT) and Swiss (SSBE) Societies for Biomedical Engineering, 8 (2), pp.89-92. ⟨10.1515/cdbme-2022-1024⟩. ⟨hal-03696320⟩
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