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Article Dans Une Revue Diagnostic Microbiology and Infectious Disease Année : 2009

Real-time polymerase chain reaction detection of Cryptococcus neoformans and Cryptococcus gattii in human samples

Résumé

We report a TaqMan® real-time polymerase chain reaction (PCR) protocol for diagnosing cryptococcosis. Specificity was tested with 33 fungal strains. The 7 clinical samples found positive by culture also tested positive by real-time PCR. No false negatives were found among the 94 clinical samples that were negative by culture for Cryptococcus spp. The sensitivity threshold was about 10 plasmid copies per assay.
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hal-03695245 , version 1 (28-06-2022)

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Vincent Veron, Stephane Simon, Denis Blanchet, Christine Aznar. Real-time polymerase chain reaction detection of Cryptococcus neoformans and Cryptococcus gattii in human samples. Diagnostic Microbiology and Infectious Disease, 2009, 65 (1), pp.69 - 72. ⟨10.1016/j.diagmicrobio.2009.05.005⟩. ⟨hal-03695245⟩

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