Fast-SEA ligation: a chemoselective NCL-inspired ligation reaction effective in the nanomolar concentration range - Archive ouverte HAL Accéder directement au contenu
Poster De Conférence Année : 2022

Fast-SEA ligation: a chemoselective NCL-inspired ligation reaction effective in the nanomolar concentration range

Résumé

We show that latent oxalyl thioester surrogates are a powerful means to modify peptides and proteins in highly dilute conditions in purified aqueous media or in mixtures as complex as cell lysates. Designed to be shelf-stable reagents, they can be activated on demand to enable ligation reactions with peptide concentrations as low as a few hundred nM at rates approaching 30 M-1 s-1.

Domaines

Chimie organique
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Dates et versions

hal-03695066 , version 1 (14-06-2022)

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Copyright (Tous droits réservés)

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  • HAL Id : hal-03695066 , version 1

Citer

Benjamin Grain, Benoît Snella, Jérôme Vicogne, Frédéric Capet, Birgit Wiltschi, et al.. Fast-SEA ligation: a chemoselective NCL-inspired ligation reaction effective in the nanomolar concentration range. GFPP 22 / BPGM 5 Joint Meeting of the French and Belgian Peptides and Proteins Societies, May 2022, Port-Leucate, France. , 2022. ⟨hal-03695066⟩
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