Effect of polystyrene nanoplastics and their degraded forms on stem cell fate - Archive ouverte HAL
Article Dans Une Revue Journal of Hazardous Materials Année : 2022

Effect of polystyrene nanoplastics and their degraded forms on stem cell fate

Gwang-Bum Im
  • Fonction : Auteur
Young Geon Kim
  • Fonction : Auteur
Tae Yong Yoo
  • Fonction : Auteur
Sung-Won Kim
  • Fonction : Auteur
Hyun Su Park
  • Fonction : Auteur
Taeghwan Hyeon
  • Fonction : Auteur
Gi-Ra Yi
  • Fonction : Auteur
Suk Ho Bhang
  • Fonction : Auteur

Résumé

Several studies have examined the effects of micro- and nanoplastics on microbes, cells, and the environment. However, only a few studies have examined their effects—especially, those of their reduced cohesiveness—on cell viability and physiology. We synthesized surfactant-free amine-functionalized polystyrene (PS) nanoparticles (NPs) and PS-NPs with decreased crosslinking density (DPS-NPs) without changing other factors, such as size, shape, and zeta potential and examined their effects on cell viability and physiology. PS- and DPS-NPs exhibited reactive oxygen species (ROS) scavenging activity by upregulating GPX3 expression and downregulating HSP70 (ROS-related gene) and XBP1 (endoplasmic reticulum stress-related gene) expression in human bone marrow-derived mesenchymal stem cells (hBM-MSCs). Additionally, they led to upregulation of MFN2 (mitochondrial fusion related gene) expression and downregulation of FIS1 (mitochondrial fission related gene) expression, indicating enhanced mitochondrial fusion in hBM-MSCs. Cell-cycle analysis revealed that PS- and DPS-NPs increased the proportion of cells in the S phase, indicating that they promoted cell proliferation and, specifically, the adipogenic differentiation of hBM-MSCs. However, the cytotoxicity of DPS-NPs against hBM-MSCs was higher than that of PS-NPs after long-term treatment under adipogenic conditions.
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Dates et versions

hal-03692493 , version 1 (09-06-2022)

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Citer

Gwang-Bum Im, Young Geon Kim, In-Seong Jo, Tae Yong Yoo, Sung-Won Kim, et al.. Effect of polystyrene nanoplastics and their degraded forms on stem cell fate. Journal of Hazardous Materials, 2022, 430, pp.128411. ⟨10.1016/j.jhazmat.2022.128411⟩. ⟨hal-03692493⟩

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