Duplex formation and secondary structure of gamma-PNA observed by NMR and CD - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Biophysical chemistry Année : 2016

Duplex formation and secondary structure of gamma-PNA observed by NMR and CD

Résumé

Peptide nucleic acids (PNAs) are non-natural oligonucleotides mimics, wherein the phosphoribose backbone has been replaced by a peptidic moiety (N-(2-aminoethyl)glycine). This peptidic backbone lends itself to substitution and the gamma-position has proven to yield oligomers with enhanced hybridization properties. In this study, we use Nuclear Magnetic Resonance (NMR) and Circular Dichroism (CD) to explore the properties of the supramolecular duplexes formed by these species. We show that standard Watson-Crick base pair as well as non-standard ones are formed in solution. The duplexes thus formed present marked melting transition temperatures substantially higher than their nucleic acid homologs. Moreover, the presence of a chiral group on the gamma-peptidic backbone increases further this transition temperature, leading to very stable duplexes. PNA duplexes with a chiral backbone present a marked chiral secondary structure, observed by CD, and showing a common folding pattern for all studied structures. Nevertheless small differences are observed depending on the details of the nucleobase sequence.

Dates et versions

hal-03680496 , version 1 (27-05-2022)

Identifiants

Citer

Justine Vieville, Sofia Barluenga Badiola, Nicolas Winssinger, Marc-André Delsuc. Duplex formation and secondary structure of gamma-PNA observed by NMR and CD. Biophysical chemistry, 2016, 210, pp.9-13. ⟨10.1016/j.bpc.2015.09.002⟩. ⟨hal-03680496⟩
22 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More