Evaluation of semi-automatic image analysis tools for cerebrospinal fluid electrophoresis of IgG oligoclonal bands - Archive ouverte HAL
Article Dans Une Revue Practical Laboratory Medicine Année : 2018

Evaluation of semi-automatic image analysis tools for cerebrospinal fluid electrophoresis of IgG oligoclonal bands

Résumé

BackgroundIgG concentrations in cerebrospinal fluid generally range from 20 to 45 mg/L. In multiple sclerosis immune reactions lead to intrathecal synthesis of specific IgGs that can be detected in biological fluid samples both quantitatively and qualitatively by isoelectric focusing of supplementary oligoclonal IgG bands.MethodA simple tool, using the MATLAB application, to facilitate and improve isoelectric focusing profile analysis is presented and evaluated in terms of its sensitivity, repeatability and reproducibility. A comparison between human readers and semi-automatic method has also been performed.ResultsResults from the semi-automatic method were found to be equivalent or superior to generally employed laboratory methods. Repeatability analysis for semi-automatic processing yielded coefficients of variation (CVs) in the 3–7% range, and using a sample with an estimated IgG concentration of 200 mg/L, four bands were still visible after dilution to 5 mg/L, corresponding to band concentrations of 1.1–1.6 mg/L. Discordances between visual inspection and automatic analysis only appear at threshold levels for interpretation (the gray zone).ConclusionThe semi-automatic method has acceptable performance for routine implementation.
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Dates et versions

hal-03678061 , version 1 (19-12-2023)

Identifiants

Citer

G. Forzy, L. Peyrodie, S. Boudet, Z. Wang, A. Vinclair, et al.. Evaluation of semi-automatic image analysis tools for cerebrospinal fluid electrophoresis of IgG oligoclonal bands. Practical Laboratory Medicine, 2018, 10, pp.1-9. ⟨10.1016/j.plabm.2017.11.001⟩. ⟨hal-03678061⟩

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