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Journal Articles Journal of The American Society for Mass Spectrometry Year : 2016

2D FT-ICR MS of Calmodulin: A Top-Down and Bottom-Up Approach

Abstract

Two-dimensional Fourier transform ion cyclotron resonance mass spectrometry (2D FT-ICR MS) allows data-independent fragmentation of all ions in a sample and correlation of fragment ions to their precursors through the modulation of precursor ion cyclotron radii prior to fragmentation. Previous results show that implementation of 2D FT-ICR MS with infrared multi-photon dissociation (IRMPD) and electron capture dissociation (ECD) has turned this method into a useful analytical tool. In this work, IRMPD tandem mass spectrometry of calmodulin (CaM) has been performed both in one-dimensional and two-dimensional FT-ICR MS using a top-down and bottom-up approach. 2D IRMPD FT-ICR MS is used to achieve extensive inter-residue bond cleavage and assignment for CaM, using its unique features for fragment identification in a less time- and sample-consuming experiment than doing the same thing using sequential MS/MS experiments.

Dates and versions

hal-03677822 , version 1 (24-05-2022)

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Federico Floris, Maria van Agthoven, Lionel Chiron, Andrew J. Soulby, Christopher A. Wootton, et al.. 2D FT-ICR MS of Calmodulin: A Top-Down and Bottom-Up Approach. Journal of The American Society for Mass Spectrometry, 2016, 27 (9), pp.1531-8. ⟨10.1007/s13361-016-1431-z⟩. ⟨hal-03677822⟩
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