Reversible electroporation (EPN) is a method for introducing molecules into cells without permanent damage based on the application of pulsed electric fields. The development of innovative in vitro assays exploiting 3D cell models such as spheroids can be of great help to assess the potential of cancer treatments based on EPN. The parallel treatment of tens of spheroids of similar characteristics (size, shape) is required to produce statistical data. To address this challenge, we designed a microfluidic platform enabling culture and electroporation of a large number of spheroids sharing similar characteristics without requiring any manipulation. Here, we demonstrate the delivery of an anti-cancer agent in spheroids using sine wave bursts.