High-throughput synthetic rescue for exhaustive characterization of suppressor mutations in human genes - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Cellular and Molecular Life Sciences Année : 2020

High-throughput synthetic rescue for exhaustive characterization of suppressor mutations in human genes

Nour Fayyad
Bassam Badran
  • Fonction : Auteur
Hussein Fayyad-Kazan
Xavier Gidrol
  • Fonction : Auteur
  • PersonId : 1136557

Résumé

Abstract Inherited or acquired mutations can lead to pathological outcomes. However, in a process defined as synthetic rescue, phenotypic outcome created by primary mutation is alleviated by suppressor mutations. An exhaustive characterization of these mutations in humans is extremely valuable to better comprehend why patients carrying the same detrimental mutation exhibit different pathological outcomes or different responses to treatment. Here, we first review all known suppressor mutations’ mechanisms characterized by genetic screens on model species like yeast or flies. However, human suppressor mutations are scarce, despite some being discovered based on orthologue genes. Because of recent advances in high-throughput screening, developing an inventory of human suppressor mutations for pathological processes seems achievable. In addition, we review several screening methods for suppressor mutations in cultured human cells through knock-out, knock-down or random mutagenesis screens on large scale. We provide examples of studies published over the past years that opened new therapeutic avenues, particularly in oncology.

Dates et versions

hal-03656568 , version 1 (02-05-2022)

Identifiants

Citer

Farah Kobaisi, Nour Fayyad, Eric Sulpice, Bassam Badran, Hussein Fayyad-Kazan, et al.. High-throughput synthetic rescue for exhaustive characterization of suppressor mutations in human genes. Cellular and Molecular Life Sciences, 2020, 77 (21), pp.4209-4222. ⟨10.1007/s00018-020-03519-6⟩. ⟨hal-03656568⟩
12 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More